大熊猫精子低渗膨胀试验与其他精子质量评估试验相关性研究

Correlation between HOST and other Methods in Evaluating Semen Quality of Giant Pandas

利用解冻后的大熊猫冷冻精液,分别进行体外存活试验、顶体反应试验、异种穿卵试验和低渗膨胀试验,并对低渗膨胀试验与其他试验作相关性分析。结果显示:各试验之间存在一定的相关性。精子低渗膨胀率与精子的体外37℃存活时间呈显著正相关,r=0.79(P<0.05);与体外异种受精率呈极显著正相关,r=0.90(P<0.01)。说明大熊猫精子的低渗膨胀试验可以作为精子质量评价的重要指标。

The correlation of methods among survival in vitro, specifically acrosome reaction, heterogenetic penetrationegg and hypoosmotic swelling test （ HOST）, of giant pandas semen was evaluated. Frozen giant panda semen was used in this research. The results were as follows： the hypoosmotic swelling rate of giant panda semen showed significant positive correlation with the survival time in vitro at 37℃, the correlation coefficient was 0.79 （P 〈 0.05 ） , the hypoosmotic swelling rate of giant panda semen showed highly significant positive correlation with the heterogenetie penetration-egg rate in vitro, the correlation coefficient was 0.90（ P 〈 0. 01 ）. The research results show that the hypoosmotic swelling test may be considered as an effective method to evaluate the semen quality of giant pandas.