条斑星鲽染色体核型分析

Study on the karyotype of Verasper moseri

采用体内注射植物血细胞凝集素(PHA)和秋水仙素法制备条斑星鲽染色体。实验鱼头肾细胞经体内短期培养,取出体外经低渗处理、卡诺氏固定液固定后,常规空气干燥法制作染色体标本。Giemsa染液染色后获得中期分裂相进行核型分析。结果表明,条斑星鲽有46条染色体,其核型为2n=46=2sm+44t,即有1对亚中部着丝点染色体和22对端部着丝点染色体,染色体臂数为NF=48,尚未发现有多倍体的现象,也未发现异型性染色体和随体染色体。

The karyotype of Verasper rnoseri was studied through the chromosome preparations obtained from head kidney by the method of injecting PHA and colchicines. After low permeating and Carnoy＇s fixation, air drying method was used to make chromosome samples, which were dyed by Giemsa afterwards. The results showed that the diploid chromosome number of Verasper moseri was 2 n=46 and its karyotype formulae were 2 n = 46 = 2 sm ＋44 t. Namely, there were one pair of submetacentric chromosome and 22 pairs of telocentric chromosome. NF=48. There was no visible evidence of polyploidy chromosome and sex chromosome.