摘要
在pBAD/gIIIA-VP37重组表达载体构建的基础上,研究发酵工艺参数对重组WSSV-VP37(rVP37)蛋白表达量的影响,并对重组质粒的稳定性和重组菌的生长进行研究。结果表明,pBAD/gIIIA-VP37表达的rVP37以包涵体形式存在于菌体中,发酵温度及诱导时间影响rVP37蛋白表达和菌体产量,以37℃诱导5h最佳。重组质粒在宿主菌中具有良好的分离稳定性,WSSV-VP37基因的插入对宿主菌的生长规律影响不大。
The effect of fermenting procedure on WSSV-VP37 expression and the stability in recombinant pBAD/gⅢA-VP37 was studied. The result showed that recombinant VP37 （rVP37） protein was expressed in inclusion body. The temperature and incubation time of fermentation affected the production of rVP37. The best results were attained by incubation for 5h at 37℃. The recombinant plasmid had excellent stability in Top10. And the study also showed that transformation of WSSV-VP37 gene had little effect on the growth of host cells.
出处
《渔业科学进展》
CSCD
北大核心
2009年第2期60-65,共6页
Progress in Fishery Sciences
基金
国家高技术发展计划项目(2006AA100312)
国家重点基础研究项目(2006CB101801)
国家自然科学基金项目(30871942)共同资助
关键词
发酵条件
重组VP37
蛋白表达
Fermenting procedure Recombinant VP37 Protein expression
