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挤压去核法和盲吸去核法在延边黄牛体细胞核移植中的对比研究 被引量:5

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摘要 分别采用挤压去核法和盲吸去核法对延边黄牛卵母细胞进行去核处理,之后进行核移植,培养观察,比较两种去核方法对延边黄牛体细胞核移植效率的影响。挤压法的操作成功率和去核率明显高于盲吸法,盲吸法操作程序耗时比挤压法长,但是差异不显著。两种方法获得了相近的卵裂率和囊胚率,但挤压法构建的重组胚在囊胚数上高于盲吸法。挤压法在延边黄牛体细胞核移植上比盲吸法更有效。
机构地区 延边大学农学院
出处 《江苏农业科学》 CSCD 北大核心 2009年第2期178-180,共3页 Jiangsu Agricultural Sciences
基金 振兴东北老工业基地项目(编号:发改高技[2004]2057号) 延边州延边大学合作项目(编号:延大科合字(2005)06号)
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  • 1Wilmut I,Schnieke A E,McWhir J,et al.Viable offspring derived from fetal and adult mammalian cells[J].Nature,1997,385:810-813.
  • 2Cibelli J B,Stice S L,Golueke P J,et al.Cloned transgenic calves produced from nonquiescent fetal fibroblasts[J].Science,1998,280:1256-1258.
  • 3Polejaeva I A,Chen S H,Vaught T D,et al.Cloned pigs produced by nuclear transfer from adult somatic cells[J].Nature,2000,407:86-90.
  • 4Lee B C,Kim M K,Jang G,et al.Dogs cloned from adult somatic cells[J].Nature,2005,436:641.
  • 5Miyoshi K,Sato K,Yoshida M.In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation[J].Cloning Stem Cells,2006,8:159-165.
  • 6Walker S C,Shin T,Zaunbrecher G M,et al.A highly efficient method for porcine cloning by nuclear transfer using in vitromatured oocytes[J].Cloning Stem Cells,2002,4:105-112.
  • 7Do minko T,Chan A,Simerly C,et al.Dynamic imaging of the metaphase Ⅱ spindle and maternal chromosomesin bovine oocytes:implications for enucleation efficiency verification,avoidance of parthenogenesis,and successful embryogenesis[J].Biol Reprod,2000,62:150-154.
  • 8Greising T,Jonas L.The influence of enucleation on the ultrastructure ofin vitro matured and enucleated cattle oocytes[J].Theriogenology,1999,52:303-312.
  • 9Nakayama T,Noda Y,Goto Y,et al.Effects of visible light and other environmental factors on the production of oxygen radicals by hamster embryos[J].Theriogenology,1994,41(2):499-510.
  • 10Robl J M,Stice S L.Prospects for the commercial cloning of animals by nuclear transplantation[J].Theriogenology,1989,31:75-84.

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  • 1王强,徐辉碧.茶叶抗脂质过氧化作用的研究[J].茶叶,1994,20(2):11-13. 被引量:6
  • 2廖鲁兴,李巧云.硒与体内酶活性及其它元素分布的关系[J].国外医学(医学地理分册),1995,16(2):53-55. 被引量:29
  • 3陈自洪,杨素芳,谢英,李日聪,韦精卫,石德顺.水牛卵母细胞去核方法的比较[J].中国兽医科学,2006,36(6):493-496. 被引量:5
  • 4罗光彬,袁水桥,李东全,文江,王冰松.添加β-巯基乙醇对牛体外受精胚胎体外发育的影响[J].河南农业科学,2006,35(9):131-133. 被引量:5
  • 5GARDNER D K, LANE M. Amino acids and ammo- nium regulate mouse embryo development in culture [J]. Biol Reprod, 1993, (48) :377-385.
  • 6DALVIT G, LLANES S P, DESCALZO A, et al. Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation[J]. Reprod Domest Anita, 2005, 40(2) :93-97.
  • 7LEYENS G,KNOOPS B,DNNAY I , et al. Expres- sion of peroxiredoxins in bovine oocytes and embryo sproduced in vitro [J]. Mol Reprod Dev, 2004, ( 11 ) : 243-251.
  • 8YERMILOV V, RUBIO J, OHSHIMA H. Forma- tion of 8-nitroguanine in DNA treated with peroxyni- trite in vitro and its rapid removal from DNA by de- purination[J]. FEBS Lett, 1995, 376(3):207-210.
  • 9EPE B, BALLMAIER D, ROUSSYN I, et al. DNA damage by peroxynitrite characterized with DNA re- pair enzymes[J]. Nucleic Acids Res, 1996, 24(21) .. 4105-4110.
  • 10ROYACK G A, NGUYEN M P, TONG D C, et al. Response of human oral epithelial cells to oxidative damage and the effect of vitamin E[J]. Oral Oncol, 2000, 36(1) ..37-41.

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