摘要
以急性早幼粒白血病人短型融合蛋白的PML与RARα结合部位的13个氨基酸序列制备合成肽,交联甲状腺球蛋白免疫Balb/c小鼠,通过杂交瘤技术获得两株单抗,经鉴定与相关蛋白无交叉反应,说明此融合部位外露,可以制备出针对性抗体。
A peptide of 13 amino acids correspoding to the short type PML-RAR a juntions was synthesized as an antigen. Peptide was coupled with thyroglobulin by MBS. By using the hybrido-ma techique we have generated two mouse monoclonal antibodies against short type PML-RARa juntion point. The isotype of both the monoclonal antibodies was IgG1 and has no cross-reaction with related protein , indicating the specificity for PML-RAR a S type fusion-point epitope.
出处
《上海免疫学杂志》
CSCD
北大核心
1998年第1期17-19,共3页
Shanghai Journal of Immunology
基金
国家自然科学基金
卫生部基金
上海血液学研究所胡应洲基金的部分资助
关键词
融合蛋白
单壳隆抗体
急性白血病
Acute promyelocytic leukemia
PML-RAR a short (S) type fusion protein
monoclonal antibody
anti-peptide antibody
