肾综合征出血热病毒对体外培养的人胚肾小球系膜细胞的影响

Effect of Hemorrhagic Fever with Renal Syndrome Virus on Human Embryo Mesangial Cells in Vitro

观察肾综合征出血热病毒Ａ９株（ＨＦＲＳＶ－Ａ９）对体外培养的人胚肾小球系膜细胞（ＭｓＣ）的影响以及病毒唑、磷甲酸钠对病毒感染的抑制作用。采用酶联免疫吸附试验（ＥＬＩＳＡ）、间接免疫荧光法（ＩＦＡ）、免疫酶组化法和电镜等技术检测ＭｓＣ中的病毒、抗原与培养上清液中的病毒滴度。结果：ＨＦＲＳＶ－Ａ９感染ＭｓＣ７天后细胞中便有病毒抗原表达；电镜下胞浆中见到病毒颗粒和包涵体；病毒唑与磷甲酸钠能降低病毒抗原表达强度和上清液中的病毒滴度，且加药时间愈早抑制作用愈强。结论：ＨＦＲＳＶ－Ａ９能感染ＭｓＣ并能在其中增殖，形成包涵体，使ＭｓＣ发生结构和功能的改变，这是导致ＨＦＲＳ急性肾衰的重要机制之一。病毒唑和磷甲酸钠能抑制ＨＦＲＳＶ－Ａ９对ＭｓＣ的感染。

To study the pathogenicity of hemorrhagic fever with renal syndrome virus A 9 strain (HFRSVA 9) to human embryo mesangial cells (MsC) in vitro and the inhibitory effect of ribavirin and foscarnet on HFRSVA 9 infection. The virus titer in culture fluid was measured by enzymelinked immunosorbent assay (ELISA). The HFRSV antigen in MsC was detected by indirect immmunofluoresence assay (IFA) and enzyme immunoassay (EIA). The shape and ultrastructure of infected MsC were observed by light microscope and transmission electron microscope (TEM) respectively. HFRSVA 9 infection of MsC was demonstrated by IFA, EIA, and ELISA on the 7th day after inoculation and the infection intensity was increased from then on. By TEM, virionlike granules and viral inclusion bodies could be found in the cytoplasm of MsC. Both ribavirin and foscarnet could inhibit the replication of HFRSV in MsC, and the earlier they were added, the stronger the viruses were affected. We conclude that HFRSV can infect MsC and replicate in it. Viral inclusion body is one mode of viral pathogenesis. MsC will change its structure and function when HFRSV invades. This may be one of the most important causes of acute renal failure of HFRS. Ribavirin and foscarnet can inhibit the replication of HFRSVA 9 strain in MsC. These data provide experimental evidence for the clinical use of antiviral treatment in the early stage of HFRS.