两株人胃腺癌裸鼠移植瘤模型的建立

Establishment of Two Implanttumor Models in Nude Mice with Human Gastric Adenocarcinoma Cell Lines

用两株人胃腺癌细胞系（ＧＣ－８０３和ＳＧＣ－７９０１）接种于裸小鼠皮下，形成初代移植瘤后鼠间传代，观察和比较了两株胃癌细胞系初代和子代移植瘤的移植成功率、生长特性和组织病理变化。结果显示：两株胃癌细胞系均能形成裸鼠皮下移植瘤，鼠间传代目前已至第１０代，总移植成功率达１００％，尚未发现移植瘤自发消退现象。比较两组移植瘤的生长速度，发现ＳＧＣ－７９０１组比ＧＣ－８０３组生长迅速。实验中未观察到裸鼠的主要脏器有转移灶形成。组织学检查表明两组移植瘤均为低分化腺癌结构，染色体分析显示两株移植瘤均具有人类恶性肿瘤细胞染色体的特点。

Two cell lines of human gastric adenocarcinomas were inoculated subcutaneously (S.C.) into nude mice to develop implanttumor models. The tumortake rates, growth characteristics and morphological structures of the two implant tumors were examind and compared. Both tumor cell lines could develop S.C. implant tumor in nude mice. The 100% of tumortake rate was obtained. These implant tumors have been maintained for over 10 generations. No spontaneous regression of the implant tumors was observed. It was found that the growth of implant tumors was more rapid in SGC7901 group than in GC803 group. No metastasis was explored in other main organs of nude mice during the experiment. Histopathological examination revealed that both implant tumors were adenocarcinomas with poor differentiation. The chromosome characteristics of human malignant neoplasm were exhibited in both implant tumors by chromosome karyotype analysis. We conclude that these two implanttumor models will be useful tools in the researches of the prevention and treatment of gastric cancer.