辐射对人红细胞酶损伤的远后效应

Delayed effects of radiation on enzymes in erythrocytes.

研究辐射对红细胞酶损伤的远后效应。方法对５例骨髓型急性放射病病人受照后第１，２，３，６年分别进行五大类代谢途径８种红细胞酶活力动态观察。结果受照后初期活力低下的酶Ｇ６ＰＤ、ＰＫ、ＭＲ及恢复期活力下降的酶ＡＣＥ在后续４次测定中均有不同程度回升，但个别患者的上述前三种酶活力始终低于正常下限。受照剂量、患者年龄与酶活力变化之间无显著相关性。结论辐射对红细胞酶损伤的远后效应在糖酵解酶ＰＫ、已糖旁路酶Ｇ６ＰＤ和氧化还原代谢酶ＭＲ表现最为明显。结果提示。

Objective\ To study the delayed effects of radiation on the enzymes in erythrocytes.Methods The activity of 8 enzymes,related glycolysis,hexose monophosphate shunt,nucleotide metabolism,redox reaction and esterase in erythrocytes of five patients with bone marrow form of acute radiation sickness(ARS) were assayed at 1,2,3 and 6 years after exposure to 60 Co radiation.\ Results \ The decreased activities of glucose 6 phosphate dehydrogenase(G6PD),pyruvate kinase(PK),NADH methemoglobin reductase (MR) during the stage of crisis and of acetylcholinesterase (ACE) during the stage of convalescence were recovered to varying extent,whereas the lowered activities of the first three enzymes in some cases remained unchanged.There was no correlation between the enzyme activity and the radiation dose as well as the age of the patients.\ Conclusion\ It is demonstrated that the delayed effects of radiation damage to erythrocyte enzymes are most significant in PK of glycolosis,G6PD of hexose monophosphate shunt and MR of redox reaction.It is suggested that the genes related to the synthesis of erythrocyte enzymes may be damaged by radiation.