骨髓型急性放射病患者红细胞膜蛋白组成与功能变化特征

Characterization of changes in composition and function of erythrocyte membrane proteins in patients with bone marrow form of acute radiation sickness.

观察辐射对红细胞膜损伤的远后效应。方法应用７．５％聚丙烯酰胺凝胶电泳法、Ｐｅｒｃｏｌ梯度密度离心、ＮＯ－２通透速率测定、ＤＩＤＳ通透抑制率测定等方法，分析５例骨髓型急性放射病（ＡＲＳ）患者受照后第６年红细胞膜蛋白组成与功能。结果辐射对膜蛋白的损伤持续存在，损伤特征为带８蛋白含量减少，带３蛋白阴离子转运功能低下。进一步分析结果显示，ＡＲＳ年轻红细胞对ＮＯ－２通透时间延迟较中、老年细胞变化明显；ＡＲＳ红细胞对ＤＩＤＳ通透抑制效应下降。结论结果提示，辐射对红细胞膜蛋白的损伤环节在骨髓红细胞合成期；ＡＲＳ红细胞膜外侧带３结合位点可能发生病变，导致阴离子转运功能下降；同时。

Objective\ The delayed effect of radiation on erythtocyte membrane protein,the composition and function of the membrane proteins in five patients with bone marrow form of acute radiation sickness(ARS) were follow up at six years after the Shanghai 60 Co irradiation accident.\ Methods \ Percoll centrifugation,SDS polyacryamide gel electrophoresis,and analysis of NO - 2 transport rate and DIDS inhibition rate were performed.\ Results \ The injuries of the membrane proteins induced by radiation,characterized by reduced content of band 8 and declined anion transport function of band 3 protein remained the same as initially observed.The further study showed that the inhibition of DIDS on the anion transport of the ARS erythrocytes was decreased and the transport time for NO - 2 by band 3 was significantly prolonged in younger erythrocytes than those in middle or old aged cells.\ Conclusion\ It is suggested that the radiation damage to erythrocyte membrane proteins might occur at the stage of erythropoiesis in bone marrow.The exofacial site in band 3 may be changed after radiation,which could result in the abnormalities in anion transport.It is believed that the aging of erythrocytes might be present in advanced stage of ARS.