摘要
目的:比较阿托品(Atr),东莨菪碱(Sco),樟柳碱(AT3)和山莨菪碱(Ani)对小鼠行为及记忆损伤作用.方法:行为和记忆实验用开阔和回避反应法.脑M受体用[3H]QNB测定.结果:Atr,Sco和AT3增加小鼠走动行为26%-42%,降低站立,修饰,排便行为50%-100%,并抑制开阔记忆.4个药物均能妨碍回避反应.小鼠18日龄额叶皮层和海马[3H]QNB结合位点数少于38日龄7%-23%.结论:1)莨菪类生物碱对小鼠行为和记忆障碍的作用随其日龄增加而减弱.2)Sco对幼年小鼠的行为及记忆障碍作用的最小有效量分别是Atr,AT3和Ani的1/10,1/100和1/1000.
AIM: To study the agerelated changes of atropine (Atr), scopolamine (Sco), anisodine (AT3), and anisodamine (Ani) on behaviors and memories. METHODS: The behaviors and memories were measured with openfield test and stepthrough task. Mcholinergic receptors were determined by quinuclidinyl benzilate (QNB). RESULTS: During acquisition session (d 1) the 18, 28, and 38dold mice pretreated with Atr, Sco, and AT3 (002, 02, 2, or 20 mg·kg-1, ip) in openfield test showed increase in walking counts by 26 %-42 %, but decrease in rearing, grooming, and defecating counts for 50 %-92 %, 67 %-100 %, and 75 %-100 %, respectively. On recall session (d 2) the walking and rearing behaviors in the 18 and 28dold mice receiving Atr, Sco, and AT3 on d 1 were higher than those in the mice receiving saline. But a lower grooming behavior on d 2 was found in the mice receiving the drugs on d 1. On d 1 Ani 20 mg·kg-1 reduced the rearing behavior by 50 % in 18dold mice and defecation by 33 %-36 % in 18 and 28dold mice. All the 4 belladonna alkaloids increased the number of avoidanceresponse errors and decreased the retention latencies in stepthrough task. Bmax of QNB binding sites in frontal cortex and hippocampus regions in the 38dold mice increased 7 % and 23 % vs in the mice of 18 d of age, respectively. CONCLUSION: 1) The effects of the belladonna alkaloids on behaviors and memories in adult mice were weaker than those in young mice. 2) The belladonna alkaloidsinduced amnesia on passive avoidanceresponse in stepthrough was more sensitive than behavioral changes and amnesia on openfield. 3) According to the lowest effective doses which insulted the behaviors or memories in young mice, Sco was about 10, 100, and 1000 times more potent than Atr, AT3, and Ani, respectively.
出处
《中国药理学报》
CSCD
1998年第2期112-116,共5页
Acta Pharmacologica Sinica
关键词
动物行为
学习
记忆
东莨菪碱
樟柳碱
山莨菪碱
animal behavior
learning
memory
brain
receptors
atropine
scopolamine
anisodine
anisodamine
belladonna alkaloids Abstract