摘要
目的:研究转染反义碱性成纤维细胞生长因子(bFGF)寡核苷酸(ODN)对培养的自发性高血压大鼠(SHR)主动脉平滑肌细胞(SMC)生长的影响.方法:用脂质体介导法将反义bFGFODN转入SMC内,用Northern杂交检测bFGF基因表达,并测定[3H]thymidine掺入和细胞计数.结果:转染反义bFGFODN(5μmol·L-1)几乎完全抑制血管紧张素Ⅱ(AngⅡ,1μmol·L-1)诱导增高的bFGFmRNA表达和明显抑制SMC增殖,在基础状态和AngⅡ刺激条件下,[3H]thymidine掺入分别被抑制265%(P<001)和420%(P<001),细胞数分别被抑制173%(P<001)和222%(P<001).结论:反义bFGFODN能有效抑制AngⅡ诱导的bFGF基因表达和SMC增殖.
AIM: To study the effect of antisense basic fibroblast growth factor (bFGF) oligonucleotides (ODN) transfection on the growth of cultured aortic smooth muscle cells (SMC) in spontaneously hypertensive rats (SHR). METHODS: Using cationic liposomemediated method, antisense bFGF ODN were introduced into SMC, bFGF gene expression was detected by Northern blotting, cell hyperplasia was evaluated by thymidine incorporation and cell counting. RESULTS: Transfection of antisense bFGF ODN (5 μmol·L-1) almost completely inhibited enhanced bFGF mRNA expression and inhibited cell proliferation induced by angiotensin Ⅱ (Ang 1 μmol·L-1 ). In basal state and Angstimulated state, thymidine incorporation was inhibited by 265 % (P<001) and 420 % (P<001) and cell number was inhibited by 17.3 % (P<001) and by 222 % (P<001), respectively. CONCLUSION: The transfection of antisense bFGF ODN into cultured SMC effectively suppressed bFGF mRNA expression and inhibited the SMC proliferation induced by Ang.
出处
《中国药理学报》
CSCD
1998年第2期132-135,共4页
Acta Pharmacologica Sinica
关键词
碱性成纤维细胞
生长因子
的义寡核苷酸
高血压
basic fibroblast growth factor
antisense oligonucleotides
hyperplasia
vascular smooth muscle
cultured cells
thymidine
angiotensin Ⅱ
inbred SHR rats