Inhibitiory effects of procainamide on rabbit platelet aggregation and thromboxane B_2 production in vitro

目的：研究普鲁卡因胺（ＰＡ）对凝血酶诱导血小板聚集和血栓素Ｂ２（ＴＸＢ２）产生的影响．方法：用比浊法和放射免疫分析法．结果：普鲁卡因胺８５，３４，１３６和５４４μｍｏｌ·Ｌ－１有明显抑制作用．抑制率分别为４５％±３７％，４８％±３２％，８８％±２３％，９２％±１５％和５３％±２４％，６５％±２６％，９０％±６％，９５％±６％．ＰＡ的浓度与血小板聚集和ＴＸＢ２产生的抑制效力之间，以及血小板聚集抑制率和ＴＸＢ２产生的抑制率之间均存在着正相关关系，三者的线性方程和主要参数为：＾Ｙ＝０２０７５Ｘ－４９１５７，ｒ＝０９９８５；＾Ｙ＝０９５４６Ｘ－３４６７２４，ｒ＝０９９２１；＾Ｙ＝０８２０２Ｘ＋１９７０６２，ｒ＝０９９２１．结论：普鲁卡因胺抑制凝血酶诱导血小板聚集和ＴＸＢ２产生．

AIM: To study the influences of procainamide (PA) on thrombin induced rabbit platelet aggregation and thromboxane B 2 (TXB 2) production in vitro . METHODS: Turbidimetry and radioimmunoassay were used. RESULTS: PA 8 5, 34, 136, and 544 μmol·L -1 inhibited thrombin induced platelet aggre￣ga￣tion and TXB 2 production, and the inhibitory rates were 45 %±37 %, 48 %±32 %, 88 %±23 %, 92 %±15 % and 53 %±24 %, 65 %±26 %, 90 % ±6 %, 95 %±6 %, respectively. There was positive correlation between PA concentration and efficiency of inhibition of platelet aggregation and TXB 2 production, and also between the inhibition % of platelet aggregation and that of production of TXB 2. The three linear equations and main parameters were =0 2075X-4 9157, r =0 9985; =0 9546X-34 6724, r =0 9921; =0 8202X+19 7062, r =0 9921. CONCLUSION: PA inhibited thrombin induced platelet aggregation and TXB 2 production in rabbits.