非聚焦超声对豚鼠耳蜗毛细胞的影响

Effects of non-focused ultrasound on the cochlea in guinea pigs

为探讨超声（Ultrasound，FU）对豚鼠耳蜗毛细胞的影响，分别以2．5MHz、8MHz非聚焦超声（NFU）照射豚鼠耳蜗3h、6h，于30min后行耳蜗毛细胞组织学及酶组织化学观察。发现照射6h组在基底膜第2周起始段0．87±0．20mm节段及基底周起始段0．80±0．20mm节段，耳蜗毛细胞琥珀酸脱氢酶（SDH）活性降低，乳酸脱氢酶（LDH）活性升高。提示：2．5MHz、8MHzNFU照射豚鼠耳蜗达一定剂量能引起不同部位耳蜗毛细胞有氧代谢改变。

Guinea pigs with normal preyer's feflex were researched for the morpholgical and the enzymatic change of cochlea under the influence of 2. 5 MHz, 8 MHz non-focused ultrasound irradiation for 3 hours or 6 hours. The results showed that no histopathalogical changes could be found. But emzyme histochemistry studies showed that 2. 5 MHz or 8 MHz non-focused ultrasound irradiaticn for 6 hours has led to enervation of SDH activity and enhancement of LDH activity of hair cells in the 0. 87 ± 0. 20 or 0. 80 ±0. 20mm areas of the second circle or the first circle respectively. The results suggest that the non-focuesd ultrasound irradiation for some dose may cause special injury of cochlear hair cells.