大鼠完全肠外营养时胆汁中8种游离脂肪酸的改变

Changes of eight free fat acids in bile of rats during total parenteral nutrition

目的：研究完全胃肠外营养（ＴＰＮ）支持时胆汁中游离脂肪酸（ＦＦＡ）的变化。方法：采用气相色谱法测定胆汁游离脂肪酸。结果：不含脂肪乳ＴＰＮ组大鼠胆汁十四碳酸（Ｃ１４∶０）、软脂酸（Ｃ１６∶０）、硬脂酸（（Ｃ１８∶０）及花生四烯酸（Ｃ２０∶４）浓度构成明显高于对照组及含脂ＴＰＮ组，而亚油酸（Ｃ１８∶２）、亚麻酸（Ｃ１８∶３）下降；含脂ＴＰＮ组胆汁中除油酸（Ｃ１８∶１）含量高于对照组外，余无异常。结论：含脂肪乳剂的ＴＰＮ可以减轻大鼠胆汁脂肪酸的异常变化，对预防ＴＰＮ时的胆道并发症将有重要意义。

Objective:To investigate the changes of 8 free fatty acids(FFA) in bile of rats during total parenteral nutrition(TPN).Methods: Measuring the FFAs in bile by gas chromotography Results:(1)Bile percentage concentrations of FFAs of C14∶0,C16∶0,C18∶0,and C20∶4,in fatfree TPN group were remarkably higher than that of controls and lipidTPN groups, but with decreased C18∶2and C18∶3,in fatfree TPN group; (2)No difference was found between controls and lipidTPN groups in percentage concentrations of FFA in bile except that C18∶1was higher than that of controls.Conclusion:LipidTPN may reduce the degree of bile FFA change during TPN, and thus play an important role in the prevention of TPNindued biliary complications.