血清浓度变化对血清荧光光谱的影响

Effect of the Change in Serum Concentration on Serum Fluorescence Spectrum

光谱分析技术应用于血液的某些疾病诊断具有方便、快速的特点。文章采用日本岛津荧光光度计RF5301,研究了血清荧光光谱随血清浓度变化的规律,为通过血液分析进行疾病诊断提供了实验依据。实验结果表明:在不同波长的激发光激励下产生的荧光光谱不同,当用220,230,310～420nm波长的光激发时,血清的荧光强度随着血清浓度的增加越来越大;而采用240～300nm波长的光激发时,血清的荧光强度随着血清浓度的增加变得越来越小。实验还发现:当采用220,230,310～420nm波长的光激发时,血清的浓度猝灭和吸收作用不明显,主要是激发出的荧光起作用;采用240～300nm波长的光激发时,血清浓度猝灭和吸收作用明显,导致荧光强度随着血清浓度的增加变得越来越弱。这对研究血清荧光光谱时血清浓度的选择具有参考价值。

The spectral analysis technology applied to some blood diseases diagnosis is convenient and speedy. The experimental result shows that the line type and peak value of the fluorescence spectra of serum excited by ultraviolet radiation with different wavelength remain the same, but the fluorescence peak value changes with the wavelength of the blazed light. The present paper studied how the serum fluorescence spectrum changes with the serum concentration, adopting Shimadzu Corporation （Japan） fluorescence photometer RF5301, and provided the experiment basis for disease diagnosis by hemanalysis. The experimental result shows that the fluorescence spectrum of serum is different under the excitation of different monochromatic light, the relative fluorescence intensity of serum increases with the serum concentration when excitated with 220,230 and 310-420 nm monochromatic light, but the serum fluorescence intensity decreases while the serum concentration increases when the excitation monochromatic light is between 240 nm and 420 nm. The experimental research found that the serum concentration quenching and absorption effect is not obvious when the experimental sample is excitated by 220, 230 and 310 to 420 um monochromatic light, and the fluorescence stimulation plays the main role. The serum concentration quenching and absorbing effect is obvious, so the fluorescence intensity becomes weaker and weaker with serum concentration increasing when the sample is excitated by 240 nm to 300 nm monochromatic light. This work provides the experimental basis for the choice of serum concentration in the study of serum fluorescence spectrum.