牛角七的体外抑菌作用研究

Antibacterial Action in vitro of Rodgersia aesculifolia Batal.

[目的]对牛角七乙醇提取物及其5个萃取部位进行体外抑菌活性研究。[方法]牛角七经乙醇提取,系统溶剂萃取分离得到5个部位。以注射用头孢曲松钠为对照,采用管碟法测定提取物及萃取部位对受试菌的抑菌活性,并测定其相应最小抑菌浓度(MIC)。[结果]牛角七提取物和乙酸乙酯部位对受试菌均有较强的抑菌活性,抑菌圈直径均大于15.00 mm,尤其提取物、乙酸乙酯和正丁醇部位对绿脓杆菌(临床株)具有较强的抑菌活性,抑菌圈直径分别达19.31、19.67和17.69 mm,其MIC均为0.6250 mg/ml。[结论]牛角七提取物及其萃取部位对受试菌种抑制作用不同,少数抑菌活性与对照药相当甚至更强。

[Objective] The study aims to research the anti-bacterial activity in vitro of the extract and its extractive fractions in Rodgersia aesculifolia Batal..[Method] Rodgersia aesculifolia Batal.is extracted by ethanol.Five fractions are obtained by systematically separating the extracts.Ceftriaxone sodium is positive control,the antibacterial activities in vitro on bacterial species are determined by cylinder-plate method and determining their corresponding minimum inhibition concentration（MIC）.[Result] The extract and ethyl acetate fractions show that all strains have high anti-bacterial activities,the diameter is bigger than 15.00 mm.Especially the extract,ethyl acetate and n-butanol fractions on Pseudomonas aeruginosa（clinic bacterial）reach to 19.31,19.67 and 17.69 mm,the MIC are 0.625 0 mg/ml.[Conclusion] The extract and its five fractions of Rodgersia aesculifolia Batal.have different anti-bacterial activities to different strains.A few of them have the same bacteriostasis as Ceftriaxone sodium for injection or even more.