奥美拉唑对胃H^(+)-K^(+)ATP酶活性及壁细胞形态影响的研究

Effect of omeprazole on gastric H^+ -K~ +. ATPase activity and parietal cell morphology

目的本文通过动物实验研究了奥美拉唑对胃Ｈ＋－Ｋ＋ＡＴＰ酶活性及壁细胞形态的影响。方法豚鼠２０只，分为两组，实验组予奥美拉唑５ｍｇ·ｋｇ－１·ｄ－１，１天２次，腹腔注射，对照组予溶媒腹腔注射，其中两对停药３天后观察。结果实验组与对照组比较，前者Ｈｔ－ＫｔＡＴＰ酶活性平均值为１．５０±２．０５／Ｌｍｏｌ·ｈｍｇ＼后者为４４．２９±２２．１３μｍｏｌ·ｈｐ＜０．００５，差异有显著性，同时壁细胞内出现分泌小管的扩张及管壁微绒毛的稀疏或失，停药３天后Ｈ＋－Ｋ＋ＡＴＰ酶活性及壁细胞形态基本恢复常态。结论奥美拉唑能抑制胃酸分泌的同时．也引起分泌小管形态明显的改变，但此作用是可逆的。

Objectives The effect of omeprazole on gastric H^+-K^+.ATPase activity and parietal cell morphology was studied on guinea pigs. Methods The guinea pigs were divided into two groups. The test group (n=10) was injected intraperition cally omeprazole (5mg/kg.wt.i.p.) twice daily for 5 days and the control group (n = 10) (weight match) with vehicle only. Results We found that the mean H -K . ATPase activity was 1.50±2.051mol.h .mg in the test group and 44.29 ±22.13μmol.h.mg-1 in the control group (P<0.005). Canalicular dilatation and loss or sparge microvilli on the canalicular wall of parietal cells were seen in the former whereas no changes were observed in the latter. The above changes returned to near normal 3 days after withdraw of omeprazole. Conclusion Hence we believe that omeprazole can obviously inhibit gastric H -K . ATPase activity and cause significant changes in parietal cell morphology, yet these changes are reversible. [