慢传输型便秘乙状结肠VIP、SP免疫组化研究

lmmunohistochemical study of VIP and SP on the colonic wall in patients with slow trentise constipation

目的探讨慢传输型便秘（ＳＴＣ）的神经病理学基础。方法应用半定量免疫细胞组织化学的方法，对１４例ＳＴＣ和１１例非梗阻性直肠腺癌患者的乙状结肠标本进行研究，主要观察肠壁内血管活性肠肽（ＶＩＰ）和Ｐ物质（ＳＰ）的变化。结果常规ＨＥ染色下，两组结肠肌间神经丛无明显改变；免疫组化见ＳＴＣ患者乙状结肠壁内ＶＩＰ含量减少（Ｐ＜０．０５）；ＳＰ含量明显降低（Ｐ＜０．００１）；而粘膜层内无明显变化。结论ＳＴＣ患者结肠壁存在明显的神经病理学变化，其结肠传输减慢可能与肠壁内ＶＩＰ和ＳＰ能神经元数量减少和／或功能障碍有关。

Objective To investigate the neuropathologic features of slow transit constipation(STC). Methods By using semi-quantitative immunohistochem- ical method, resected sigmoid colon spectimens from 14 patients with STC and 11 non-obstructed rectal adenocarcinoma controls were investigated with rabbit anti-substance P and rabbit anti-vasoactive intestinal peptide antibody. Results Hematoxylin and eosin staining showed no changes in the colonic myenteric plexus of the two groups, ln patients with STC the mean content of vasoactive intestinal peptide(VIP) in the myenteric plexus was . lower than that of the controls (P<0.05).Substance P (SP) levels in the myenteric plexus of STC was reduced signifieantly when compared with the control tissues (P<0.001). There was no marked difference of VIP and SP content in the mucous. Conclusion There was marked neuropathologic features on the colonic wall in patients with STC. The colonic slow transit is probablly associated with the decrease of VIP and SP in the Coionic Wall.