复方瑞康欣胶囊对吗啡依赖戒断大鼠焦虑行为及海马突触素表达的影响

Effects of Compound Ruikangxin Capsula on anxiety-like behavior and relative expression of hippocampal synaptophysin in morphine-withdrawal rats

目的观察复方瑞康欣对吗啡依赖戒断大鼠焦虑行为的影响，并探讨其作用机制。方法健康雄性SD大鼠，剂量递增法sc吗啡10d，确认吗啡依赖模型建立成功后，在吗啡自然戒断的1～3d，复方瑞康欣100、200、300mg／kg和丁螺环酮15mg／kg ig治疗（2次／d），于末次吗啡注射后72h．高架十字迷宫实验评价大鼠的焦虑水平，免疫组化检测各组大鼠海马突触素的表达。结果与生理盐水（NS）治疗组比较，复方瑞康欣200、300mg／kg和丁螺环酮治疗能明显增加大鼠进入开臂次数和时间的比例（P〈0．01、0．05），同时海马突触素的表达显著降低（P〈0．01）。结论复方瑞康欣对吗啡依赖大鼠戒断后的焦虑治疗效果明显；逆转焦虑大鼠海马增高的突触索／突触，可能是复方瑞康欣临床治疗吗啡戒断后焦虑的机制之一。

Objective To observe effect of Compound Ruikangxin Capsula （CRC） on anxiety-like symptoms of morphine-withdrawal rats and investigate its mechanism. Methods The elevating doses were applied to validating anxiety-like behavior in SD male rats with sc morphine for 10 d. Treatments with ig administration of CRC （100, 200, and 300 mg/kg） and buspirone （15 mg/kg） were performed during 1- 3 d morphine withdrawal （twice every day）. The plus elevated maze was applied to validating anxiety-like symptom in rats. The expression levels of synaptophysin were detected on hippocampus in groups by immunohistochemitry 72 h after sc morphine. Results As compared with the control group, animals treated with CRC （200 and 300 mg/kg） and buspirone had higher ratio of entry into open arm （P〈0.01）, as well as time of remaining in open arm （P〈0.05）. At that, lower level of synaptophysin was measured on hippocampus from those rats （P〈0.01）. Conclusion CRC would play an important role in rehabilitating the anxiety like behavior in morphine-withdrawal rats. Reversal of synaptophysin on hippocampus of anxious rats could be contributed to exploring the mechanism.