shRNA-CXCR4对人乳腺癌细胞侵袭力的影响

The Effects of shRNA-CXCR4 on Breast Cancer Cells Migration and Invasion

目的研究重组质粒shRNA-CXCR4对人乳腺癌细胞侵袭潜力的影响。方法通过构建重组质粒shRNA-CXCR4沉默CXCR4基因后,运用RT-PCR,Western-blot检测3株人乳腺癌细胞的CXCR4mRNA及其蛋白的表达;建立体外侵袭模型,测定人乳腺癌细胞株穿透Matrigel的潜力。结果质粒shRNA-CXCR4作用于3株人乳腺癌细胞后能明显抑制其CXCR4基因的mRNA及蛋白表达水平(P<0.05);3株人乳腺癌细胞穿透Matrigel的能力明显降低(P<0.05)。结论shRNA-CXCR4后能明显降低人乳腺癌细胞的侵袭潜力。

Objective To study the effect of shRNA-CXCR4 on human breast cancer cells migration and invasion. Methods Through knockdown CXCR4 by shRNA, the CXCR4 mRNA expression and protein expression were examined with RT-PCR and Western blot separately. The matrigel penetrating ability of human breast cancer cells was examined in vitro experiments. Results The CXCR4 mRNA express and its protein express decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells （P〈0.05）. The invasive ability of human breast cancer cells was significantly decreased （P〈0. 05）. Conclusion through knockdown CXCR4 by shRNA, the invasive ability of human breast cancer cells can be inhibited obviously.