缺血预处理与氧化苦参碱对肺缺血再灌注损伤保护的实验研究

An Experimental Study of Protective Effects of Ischemic Preconditioning and Oxymatrine on Lung Ischemia Reperfusion Injury

目的探讨缺血预处理(ischemic preconditioning,IPC)与氧化苦参碱(oxymatrine,OMT)对肺缺血再灌注损伤(lungischemia reperfusioninjury,LIRI)的保护作用及机制。方法24只大白兔随机分为假手术组(sham组,n=6);缺血再灌注损伤组(ischemic reperfusion,I/R,n=6);IPC组(n=6);IPC+OMT组(n=6)。测定各组开胸后0min,缺血后40min,再灌注后80min、120min、160min各时点肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、湿干重比(W/D)、凋亡指数(apoptosis index,AI)、热休克蛋白90α(heat shock protein90α,Hsp90α)表达及160min时肺病理改变。结果各组开胸后0min、缺血后40min时SOD、MDA值差异无统计学意义(P>0.05),I/R80min后,I/R组W/D、AI值高于IPC、IPC+OMT组(P<0.05,P<0.01),SOD、Hsp90α表达低于IPC、IPC+OMT组(P<0.05,P<0.01)。IPC+OMT组与IPC组比较,MDA、W/D、AI低于IPC组(P<0.05);SOD、Hsp90α表达持续上调(P<0.05)。IPC组和IPC+OMT组肺病理改变较I/R组明显减轻。结论IPC与OMT诱导肺组织SOD和Hsp90α的高表达,减少MDA的生成,抗氧化损伤,抑制细胞凋亡,对减轻LIRI有协同作用。

Objective To investigate the protective effects of ischemic preconditioning（IPC） and oxymatrine （OMT） on lung ischemia reperfusion injury（LIRI）in rabbit. Methods Animal models of LIRI in rabbit were used. Twenty four rabbits were randomly divided into 4 groups： Group sham（n=6） ,Group I/R （isehemie reperfusion, n =6）, Group IPC （n = 6）, Group IPC plus OMT （n =6）. Lung tissue samples were collected at 0 min after thoraeotomy,40 min after lung isehemia and 80 min, 120 min, 160min after lung reperfusion. SOD（Superoxide Dismutase）, MDA（malondialdehyde）, Hsp90α （ Heat Shock Protein 90α）, AI （Apoptosis Index）, W/D （Wet/Dry weight ratio） and histology of lung by light microscope were studed in each group. Results SOD,MDA in Groups sham,I/R,IPC and IPC plus OMT were not significantly different at 0 min,40 min（P〉0. 05）. AI and W/D in Group I/R were significantly higher than that of Groups IPC and IPC plus OMT at 80 min after LIR（P〈0.05 ,P〈0.01） ,SOD activity and the expressions levels of Hsp90α were significantly lower than that of Groups IPC and IPC plus OMT （P〈0.05,P〈0.01） ,and MDA,W/D,AI in Group IPC plus OMT were significantly lower than that of Group IPC （P〈0.05）, but the expressions levels of SOD and Hsp90α were significantly higher than that of Group IPC（P〈0.05）. The lung tissue injury in Group IPC plus OMT was less than Groups IPC and I/R. Conclusion IPC and OMT can protect against LIRI in rabbits by decreaseing the levels of MDA and inereasesing the expressions of SOD and Hsp90α, and inhibiting the apoptosis of alveolar cells after LIRI.