VEGF和NGF在人脑胶质瘤的表达变化

Expression of VEGF and NGF in Gliomas of Human

目的研究血管内皮细胞生长因子(VEGF)和神经生长因子(NGF)在胶质瘤内的表达,探讨它们与胶质瘤病理分级的关系及其在胶质瘤发生过程中的作用。方法通过免疫组织化学SP法和免疫印迹技术检测5例正常脑组织和20例胶质瘤中VEGF和NGF的表达。结果VEGF和NGF在胶质瘤及正常脑组织内均有表达。VEGF主要分布于胶质瘤细胞的细胞浆,而NGF在胶质瘤细胞胞核和胞浆均有表达。免疫印迹结果显示:VEGF和NGF在胶质瘤中的蛋白水平均高于正常脑组织,并且随着胶质瘤病理级别(Ⅰ～Ⅳ)的增高蛋白表达逐渐增加,差异均有统计学意义(P<0.05)。结论VEGF和NGF在胶质瘤内的表达均高于正常脑组织,且随着病理级别的增高而增加,提示VEGF和NGF在胶质瘤的发生过程中可能起重要的作用,其水平高低与胶质瘤的恶性程度有关。

Objective To examine the expression of vascular endothelial cell growth factor（VEGF） and nerve growth factor （NGF） in gliomas of human, and investigate the relationship between VEGF, NGF and pathologic grading as well as tumorigenesis of gliomas. Methods Immunohistochemistry （SP） and Western blot were applied to evaluate the expression of VEGF and NGF in 5 cases of normal controls and 20 cases of human gliomas. Results VEGF and NGF were expressed in both normal brain tissues and gliomas. VEGF was observed mainly in the cytoplasm of gliomas, while NGF were occurred in both cytoplasm and nucleus of gliomas. Western blot analysis demonstrated that the expression level of VEGF and NGF was significantly higher than that in normal controls （P〈 0.05）, and a significant difference in vary pathologic grading gliomas was also observed. Conclusion VEGF and NGF were expressed in both the normal brain tissues and gliomas. The level of VEGF and NGF in gliomas was upregulated than that of the normal controls. The results indicate VEGF and NGF may play an important role in the tumorigenesis of gliomas, and relate to the extent of gliomas malignant.