摘要
目的研究天然植物银杏酸对喉癌Hep-2细胞生长的抑制作用,并探讨其作用机理。方法采用四唑盐比色实验(MTT)、DNA电泳、流式细胞仪分析等方法观察不同浓度和不同作用时间银杏酸对喉癌Hep-2细胞增殖和凋亡的影响。结果银杏酸对喉癌Hep-2细胞呈现时间和剂量依赖性的生长抑制作用;DNA经琼脂糖电泳可见典型的梯形条带;流式细胞仪分析显示银杏酸作用后,细胞凋亡率呈时间和剂量依赖性增加。结论银杏酸可抑制肿瘤细胞生长、诱导肿瘤细胞凋亡,具有明显的体外抗肿瘤活性。
Objective To investigate the anti-cancer effects and mechanism of natural plant ginkgolic acids (GAs) on Human Hep-2 cancer cells. Methods Hep-2 cancer cells were treated with different concentration of GAs for different times. The proliferation of Hep-2 cancer cells were detected by MTT colorimetric assay. Biochemistry characteristics of cell apoptosis was analyzed by DNA agarose electrophoresis and FCM. Results The proliferation of Hep-2 cancer cells were inhibited significantly by GAs in a dose and time-dependent manner. Typical DNA ladder bands of Hep-2 cell DNA treated with GAs were observed on agarose electrophoresis gels. Increased apoptosis of Hep-2 cells treated with GAs was also noticed with FCM analysis. Conclusion Ginkgolic acids show an anti-tumor activity through inhibition the growth and inducing apoptosis of tumor cell in vitro.
出处
《四川大学学报(医学版)》
CAS
CSCD
北大核心
2009年第3期459-461,共3页
Journal of Sichuan University(Medical Sciences)