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天然植物银杏酸诱导喉癌Hep-2细胞凋亡的初步研究 被引量:3

Effects of Natural Plant Ginkgolic Acids on the Apoptosis of Human Hep-2 Cancer Cells
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摘要 目的研究天然植物银杏酸对喉癌Hep-2细胞生长的抑制作用,并探讨其作用机理。方法采用四唑盐比色实验(MTT)、DNA电泳、流式细胞仪分析等方法观察不同浓度和不同作用时间银杏酸对喉癌Hep-2细胞增殖和凋亡的影响。结果银杏酸对喉癌Hep-2细胞呈现时间和剂量依赖性的生长抑制作用;DNA经琼脂糖电泳可见典型的梯形条带;流式细胞仪分析显示银杏酸作用后,细胞凋亡率呈时间和剂量依赖性增加。结论银杏酸可抑制肿瘤细胞生长、诱导肿瘤细胞凋亡,具有明显的体外抗肿瘤活性。 Objective To investigate the anti-cancer effects and mechanism of natural plant ginkgolic acids (GAs) on Human Hep-2 cancer cells. Methods Hep-2 cancer cells were treated with different concentration of GAs for different times. The proliferation of Hep-2 cancer cells were detected by MTT colorimetric assay. Biochemistry characteristics of cell apoptosis was analyzed by DNA agarose electrophoresis and FCM. Results The proliferation of Hep-2 cancer cells were inhibited significantly by GAs in a dose and time-dependent manner. Typical DNA ladder bands of Hep-2 cell DNA treated with GAs were observed on agarose electrophoresis gels. Increased apoptosis of Hep-2 cells treated with GAs was also noticed with FCM analysis. Conclusion Ginkgolic acids show an anti-tumor activity through inhibition the growth and inducing apoptosis of tumor cell in vitro.
出处 《四川大学学报(医学版)》 CAS CSCD 北大核心 2009年第3期459-461,共3页 Journal of Sichuan University(Medical Sciences)
关键词 银杏酸 喉癌HEP-2细胞 细胞增殖和凋亡 Ginkgolic acids Hep-2 cell Proliferation and apoptosis
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  • 1Kuribara H, Weintraub ST, Yoshihama T, et al. An anxiolyticlike effect of Ginkgo biloba extract and its constituent, ginkgolide-A, in mice. J Nat Prod,2003,66(10):1333-1337.
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  • 5杨小明,钱之玉,陈钧,朱伟,谢吉民.银杏外种皮中银杏酸的体外抗肿瘤活性研究[J].中药材,2004,27(1):40-42. 被引量:27

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