鼻咽癌患者血浆sCD40L,sP-selectin和sICAM-1的表达及其意义

sCD40L,sP-selectin and sICAM-1 Plasma Levels in Nasopharyngeal Carcinoma

目的探讨可溶性CD40配体(sCD40L)、可溶性P-选择素(sP-selectin)和可溶性细胞间黏附分子-1(sICAM-1)在鼻咽癌患者血浆中的表达及临床意义。方法收集110名鼻咽癌患者和55名年龄和性别匹配的健康成人的血浆,用酶联免疫吸附法(ELISA)测定sCD40L、sP-selectin和sICAM-1在血浆中的表达。结果鼻咽癌患者血浆sCD40L、sP-selectin和sICAM-1含量明显高于对照组,颈淋巴结转移者高于非转移者,且sCD40L和sP-selectin在鼻咽癌患者治疗后的表达明显低于治疗前,而sICAM-1只在Ⅲ+Ⅳ期鼻咽癌患者治疗后的表达有下降。结论鼻咽癌患者血浆sCD40L、sP-selectin和sICAM-1存在高表达现象,并且sCD40L、sP-selectin和sICAM-1在鼻咽癌的转移和疗效判断上有一定的价值。

Objectives To analyze plasma levels of soluble CD40 ligand （sCD40L）, soluble P-selectin （sP- selectin） and soluble intercellular adhesion molecule-1 （sICAM-1）in the patients with nasopharyngeal carcinoma （NPC）. Methods Plasma samples were obtained from 110 NPC patients and 55 age and sex matched healthy individuals as controls. Using commercially available enzyme-linked immunosorbent assay （ELISA） kits, plasma levels of sCD40L, sP-selectin and sICAM-1 were measured in all the samples. Results The plasma levels of sCD40L, sP-seleetin and sICAM-1 in NPC patients were higher than those in the controls, while the levels of these three proteins in NPC patients with neck node metastasis were significantly higher than those in the patients without metastasis. Moreover, plasma sCD40L and sP-selectin levels in the patients were significantly decreased after treatment, and sICAM-1 levels decreased only in stage Ⅲ ＋ Ⅳ patients. Conclusions The levels of sCD40L, sP- selectin and sICAM-1 increased in NPC patients, especially in those with neck node metastasis. Abnormal high plasma levels of these molecules may be related to metastasis, which might be used as therapeutic effect indicators in NPC patients.