不同病理类型臂丛损伤后变性神经中胶原的变化

Immunohistochemical and ultrastructural study of collagen in degenerating nerves after different pathological brachial plexus injuries

目的研究不同病理类型的臂丛损伤后变性神经中胶原蛋白的变化规律。方法建立ＳＤ大鼠不同病理类型的臂丛损伤模型，用免疫组织化学和电镜方法，分别检测变性的远端神经中Ｉ型蛋白含量的变化及观察胶原纤维的超微结构。结果胶原含量在节前组中术后６个月组略上升，节后组术后１个月组即开始上升，３个月以上上升显著。节后组胶原含量多于节前组（Ｐ＜０．０１）。

Objective Immunohistochemical and ultrastructural study was applied to explore the changes of collagen in degenerating nerves after different pathological brachial plexus injuries. Methods 80 SD rats were randomly divided into two groups, one being right C 5, C 6 preganglionic injury, the other postganglionic injury. Samples of postoperative 2 weeks, 1 month, 2 months, 3 months and 6 months were tested respectively for their type I collagen protein content. Ultrastructure of the collagen fibers was studied in the meantime. Results Among the preganglionic injuries, a slight increment of collagen content was found in 6 months' group. The collagen content began to increase one month postoperatively in the nerves of postganglionic injuries, and a significant rise was seen since the 3rd postoperative month. Conclusion The increment of collagen content in the nerves of preganglionic injury was slower than in those of postganglionic injury.