动脉硬化性血栓性脑梗塞与载脂蛋白B基因XbaI、EcoRI多态的相关性研究

The restriction fragment length polymorphism(XbaI,EcoRI) of apolipoproteinB gene in relation to the atherothrombotic cerebrol infarction

应用聚合酶链反应（ＰＣＲ）技术研究了８４例动脉硬化性血栓性脑梗塞（ＡＴＣＩ）患者和１０７例正常人的载脂蛋白Ｂ（ａｐｏＢ）基因的ＸｂａＩ、ＥｃｏＲＩ位点的多态性。结果ＡＴＣＩ组Ｘ－频率（０．８２７）低于对照组（０．９１６），ＡＴＣＩ组Ｘ＋Ｘ－基因型频率增高，与对照组比较有显著性差异，而且Ｘ＋Ｘ－基因型与血脂水平增高有相关性。提示，ａｐｏＢ基因的ＸｂａＩ多态及连锁失衡可能作为动脉粥样硬化的遗传背景影响血脂质代谢，并参与ＡＴＣＩ的发病机制。

The polymorphic sites of the apolipoproteinB gene(apoB),the XbaI,EcoRI sites was examined by the polymerase chain reaction(PCR) technique in a sample of 84 patients with atherothrombotic cerebral infarction(ATCI) and 107 healthy agematched controls.Allele frequencies were compared between cases and controls,and their impact on lipid matabolism was also studied.The frequencies of X-alleles in ATCI cases were found as 0.827,which was lower than 0.916 in controls,and the differencies reached significance.Futhermore,the frequencies of X+X-genotypes(0.345) in group of ATCI cases was higher than 0.168 in controls,and the X+X-genotype associated with higher levels of Tch,LDLch,apoB.The date suggested that the variation in linkage disequilibrium with the XbaI of the apoB gene as a genetic background of atherosclerosis,may have correletion with the determination of lipid and apoB,and may contributed to the development of ATCI.