摘要
目的大鼠吸入一定时间的高浓度氧后,测定其脑组织匀浆内活性氧含量的变化,评价吸入高浓度氧的安全性。方法选用纯种wister大鼠60只,随机分为A、B、C、D、E5组,每组12只,其中A、B、C、D组为高氧组,分别放置于氧浓度为85%~100%的自制氧箱中,于实验4、8、12、16h后制取标本,E组为空气对照组。取脑组织低温高速粉碎、离心提取匀浆液,比色法测定脑组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、一氧化氮合酶(NOS)、谷胱甘肽(GSH)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)的浓度。结果与对照组相比,高氧各组脑组织匀浆中MDA、NO、NOS均显著升高(P<0.01?,并随吸氧时间的延长有升高的趋势;而SOD、GSH、CAT、T-AOC均显著降低(P<0.01?,并随吸氧时间的延长有降低的趋势。结论吸入高浓度氧导致脑内活性氧浓度的升高,应引起重视。
Objective To evaluate the security of inspiring high concentration oxygen by detecting the reactive oxygen species of the rats brain homogenates after inspiring high concentration oxygen for a certain time. Methods Sixty wister rats weighting 150 - 200g were randomly devided into five groups( the group of 4h.8h,12h,16h and the control group,12/group). The experimental rats were exposed to 85% - 100% oxygen in a self - made chamber and the control animals were kept in normal room air. The Concentration of the MDA, SOD, GSH, NO,NOS,CAT and T - AOC of the brain homogenates was detected. Results Compared with the control group, the level of MDA, NO and NOS in the brain homogenates grew up markedly(P 〈0.01 ) and SOD,GSH,CAT,T - AOC fell down accordingly(P 〈0.01 ). Conclusion Inspiring high concentration oxygen can make the reactive oxygen species grown up, and we should pay attention to the resuit.
出处
《医学研究杂志》
2009年第5期99-101,共3页
Journal of Medical Research
关键词
高浓度氧
脑组织匀浆
活性氧
High concentration oxygen
Brain homogenates
Reactive oxygen species