阿霉素对大鼠肺缺血再灌注损伤的保护作用

Protective effects of adriamycin against lung ischemia-reperfusion injury in rats

目的观察阿霉素对大鼠肺缺血再灌注损伤(LIRI)的保护作用及其可能的机制。方法建立大鼠在体肺缺血再灌注模型。将健康Wistar大鼠54只随机分为手术对照组(C组)、缺血再灌注组(IR组)、阿霉素组(A组)。每组分别在缺血45min,再灌注60、120min3个时点处死6只大鼠,留取血浆和右肺组织,测定血清丙二醛(MDA)含量、肺组织干湿质量比值(D/W)、髓过氧化物酶(MPO)活性及肺组织中细胞凋亡指数(AI),并进行肺组织病理学检查。结果再灌注期间IR组MPO活性、AI、血清MDA升高,D/W降低,而预先给予阿霉素干预后可以减弱缺血再灌注诱导上述指标的变化。肺组织病理学检查显示阿霉素预先给药减轻肺缺血再灌注损伤。结论阿霉素对肺缺血再灌注损伤有保护作用,可能与其抑制氧自由基生成、中性粒细胞浸润及细胞凋亡有关。

Objective To investigate the protective effects of adriamycin on lung ischemia-reperfusion injury in rats and its possible mechanisms. Methods Rat models of lung ischemia-reperfusion were established. Fifty-four healthy Wistar rats were randomly divided into three groups： surgical controls （C group）, isehemia-reperfusion group OR group） and adriamyein-pretreatod group （A group）. At each of three time spots （45min from isehemia, 60 rain and 120min from reperfusion）, every 6 rats from the groups were sacrificed, collected for plasma sample and the whole fight lung. The plasma contents of malondialdehyde （MDA）, the dry/wet （D/W） weight ratio of the lung, activity of myeloperoxidase （MPO）, and apoptotic index （AI） in lung tissue were determined. Histopathological study of the lung tissue was also performed. Results The plasma contents of MDA, MPO activity and AI in lung tissue were all significantly increased during reperfusion in IR group, while the D/W ratio of lung were lower than those in the other groups. Pretreatment with adriamycin was found to attenuate the abnormality in these parameters induced by IR. Pathological examination showed that the IR induced injury was also ameliorated with adriamycin pretreatment. Conclusion Adriamycin treatment before ischemia appeared to protect the lungs against IR induced injury, possibly by inhibited PMN infiltration, reduced production of oxygen radicals and suppressed apoptosis of lung tissue.