贵州省地方性氟中毒地区人群GSTP1基因Ile105Val位点多态性分析

Analysis of the GSTP1 gene Ile105Val polymorphism in fluorosis areas in Guizhou Province

目的探讨贵州省毕节市燃煤型氟中毒地区人群谷胱甘肽硫转移酶（GSTs）活性及GSTP1基因Ile105Val位点多态性。方法在贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取160名村民作为非干预组，在毕节市长春镇燃煤型氟中毒改灶村抽取153名村民作为干预组，在非病区长顺县白云山镇抽取151名村民作对照组，用比色法分别测定各组GSTs活力；PCR-限制性片段长度多态性（PCR—RFLR）法检测各组GSTPl基因Ile105Val位点多态性[野生纯合型（AA）、突变杂合型（AG）、突变纯合型（GG）]。结果GSTs活力组间比较差异有统计学习意义（F=51．71，P〈0．05）。其中对照组[（24．30±6．27）kU／L]高于干预组[（20．78±6．20）kU／L]、非干预组[（12．44±4．97）kU／L]，组问两两比较差异有统计学意义（P均〈0．05）；同组性别间比较差异无统计学意义（P均〉0．05）。GSTP1基因Ile105Val位点多态性，干预组[AA：67．3％（103／153），AG：29．4％（45／153），GG：3．3％（5／153）]、非干预组[AA：66．9％（107／160），AG：30％（48／160），GG：3．1％（5／160）]与对照组[AA：74．8％（113／151），AG：25．2％（38／151），GG：0（0／151）]比较，差异有统计学意义（X2=6．04、6．07，P均〈0．05）；干预组与非干预组比较，差异无统计学意义（X。：0．02，P〉0．05）。结论氟中毒能导致机体GSTP1基因Ile105Val基因位点的多态性发生变化，人为干预对氟的摄入可改善氟对机体的影响。

Objective To investigate plasma glutathione S-transferase （GSTs） activity and GSTPI gene Ile105Val polymorphism in Bijie City, Guizhou Province, a coal-burning fluorosis endemic area. Methods One hundred and sixty villagers from Yachi Twon using non-improved cooking stoves were selected as the non-intervened group in Bijie City, Guizhou Province where coal-burning fluorosis was prevailing; 153 villagers as the intervented group were chosen from Changchun Twon, where cooking stoves were improved; 151 villagers were served as the control group from Baiyunshan Twon i Changshun County without endemic fluorosis. The activity of GSTs was tested by colorimetric analysis with spectrophotometer. The genotype of the GSTP1 gene Ile105Val polymorphism, presenting as either homozygous wild-type （AA）, or heterozygous mutation type （AG）, or homozygous mutation type （GG）, was detected through the PCR-RFLP procedure. Results The activity of GSTs in plasma of non-intervened group[ （12.44 ± 4.97）kU/L] was significantly lower than that of intervened group（P 〈 0.05）, and that of intervened group[ （20.78 ± 6.20）kU/L] was significantly lower than that of control group [（24.30± 6.27）kU/L, P 〈 0.051- The difference of the enzyme activity of three groups were statistically significant （F = 51.71 ,P 〈 0.05 ）, but this enzyme activity did not vary significantly in each sex of each group（P 〉 0.05 ）. Compared intervened group [ AA ： 67.3% （ 103/153 ）, AG ： 29.4% （45/153 ）, GG ： 3.3% （5/153 ） ] and non-intervened group [AA：66.9%（107/160）,AG ： 30% （ 48/160 ）, GG ： 3.1% （ 5 / 160 ） ] with control group [ AA ： 74.8 % （ 113 / 151 ）, AG ： 25.2% （ 38/151 ）, GG ： 0 （0/151） ], the Ilel05Val polymorphism site of GSTP1 gene had significant difference（xz = 6.04,6.07, both P 〈 0.05）, but not significant between intervened and non-intervened groups（X2 = 0.02, P 〉 0.05）. Conclusions Fluorosis can decrease the activity of GSTs and introduce the GSTP1 gene Ile105Val polymorphism, intervention with the fluorine intake will improve the effect of fluoride on the body.