多房棘球绦虫混合重组BCG—EmⅡ／3和BCG-Em14—3—3疫苗免疫小鼠后IgG及其亚类和IgE的动态观察

Dynamic observation on IgG and its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3. and BCG-Em14-3-3 vaccine of Echinococcus multilocularis

目的动态观察多房棘球绦虫混合重组BCG—EmⅡ／3和BCG—Em14—3—3疫苗免疫小鼠后IgG及其亚类和IgE的变化。方法12～14周龄、体质量20～25g的雌性Balb／c小鼠40只，鼻腔内接种上述疫苗，在0、2、4、6、8、10、12、14、16和18周按体质量随机剖杀4只小鼠，经眼球取血，常规酶联免疫吸附试验法测定血清中IgG及其亚类和IgE水平。结果Balb／c小鼠的血清IgG、IgG2a和IgG2b水平均在免疫后第2～18周升高，并分别在免疫后第10、4和4周达到最高水平，其值分别为0．095±0．033、0．022±0．001和0．023±0．003，与0周（0．030±0．013、0．012．4-0．004和0．013±0．004）比较，差异有统计学意义（q值分别为2．95、4．87和2．81，P〈0．05或〈0．01）；血清IgG1、IgG3和IgE水平均在免疫后2～18周降低，并分别在免疫后4、2和6周达较低水平，其值分别为0．03±1．004、0．136±0．002和0．114±0．002，与0周（0．192±0．007、0．175±0．013和0．024±0．003）比较，差异有统计学意义（q值分别为5．16、4．93和5．32，P〈0．05或〈0．01）。结论多房棘球绦虫混合重组BCG-EmⅡ／3和BCG--Em14—3—3疫苗在免疫早期即可诱导小鼠产生辅助性T细胞（TH）Ⅰ型保护性免疫反应。

Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Eml4-3-3 vaccine of Echinococcus multilocularis （Em）. Methods Forty Balb/c mice of 12 - 14 week old and 20 - 25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and lgG2b in the sera of mice increased obviously on 2 - 18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095 ± 0.033,0.022 ± 0.001,0.023 ± 0.003 respectively, as compared with the value on 0 week（0.030± 0.013,0.012 ± 0.004,0.013 ± 0.004）, the difference being statistically significant（q = 2.95,4.87,2.81 respectively, P 〈 0.01 or P 〈 0.05）; levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2 - 18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031± 0.004,0.136 ± 0.002,0.114 ± 0.002 respectively, as compared with the value on 0 week（0.192 ± 0.007, 0.175 ± 0.013,0.024 ±0.003）, the difference being statistically significant （q =5.16,4.93,5.32 respectively, P 〈 0.01 or P 〈 0.05）. Conclusion Helper T cell（TH） Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.