克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Experimental study on cardiac pathological change in rats fed with corn and bean puree of Keshan disease area

目的探讨克山病病区玉米及酱块与大鼠心肌损伤的关系。方法将雄性Wistar大鼠按体质量随机分为3组，分别饲以病区玉米、病区玉米＋酱块、非病区玉米，l、3个月时检测大鼠尾静脉全血谷胱甘肽过氧化物酶（GSH—Px）活力，6个月时光镜下观察大鼠心肌病理改变。结果1、3个月时3组大鼠全血GSH～Px活力比较，差异有统计学意义（F=23．60、72．46，P均〈0．01），其中病区玉米组[（181．58±22．15）、（44，76±28．59）U／L]和病区玉米＋酱块组[（195．03±17．66）、（30．38±3．35）U／L]明显低于非病区玉米组[（340．90±95．42）、（125．17±13．64）U／L，P均〈0．01]，而病区玉米组与病区玉米＋酱块组比较，未见明显改变（P〉0．05）。光镜下3组大鼠心肌损伤病理改变发生率分别为3／9、1／9、2／7，组间比较差异无统计学意义（P〉0．05）。结论．单纯克山病病区玉米饲养可引起大鼠心肌损伤病理性改变，酱块无加重心肌损伤作用。

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in I and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in I and 3 months respectively（F = 23.60,72.46, all P 〈 0.01 ） ; GSH-Px activity was （181.58 ± 22.15）, （44.76 ± 28.59）U/L in rats fed with corn, was （195.03 ±17.66）, （30.38 ± 3.35）U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic areal （340.90 ± 95.42）, （125.17 ± 13.64）U/L, all P 〈 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious（P 〉 0.05）. Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance（X2= 1.33, P 〉 0.05）. Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.