电针预处理对脑缺血再灌注大鼠骨髓和血浆中EPCs及VEGF的影响

The influence of electroacupuncture pretreatment to endothelial progenitor cells and vessel endothelial grouth factor in marrow and peripheral blood of cerebral ischemia-reperfusion rats

目的:观察电针预处理对脑缺血再灌注损伤大鼠骨髓和外周血中内皮祖细胞(EPCs)、血管内皮生长因子(VEGF)的影响,研究电针预处理减轻脑缺血再灌注损伤机制。方法:将大鼠用抽签法随机分组,分为治疗组、模型组、假手术组和正常对照组。将大鼠造模脑缺血再灌注后,在第12,24,48小时分别将各组大鼠留取标本,用流式细胞仪检测其骨髓和外周血中EPCs的百分比变化。用ELISA法检测骨髓和血清中VEGF的浓度。结果:脑缺血再灌注损伤的大鼠较正常大鼠的骨髓和外周血中EPCs和VEGF的含量增加。电针预处理过的脑缺血再灌注损伤大鼠较未经过预处理的大鼠的骨髓和外周血中EPCs和VEGF的含量增加;随着电针预处理后时间的延长,EPCs和VEGF的含量增加越多。结论:电针预处理可以通过提高脑缺血再灌注损伤大鼠骨髓和外周血中VEGF的浓度,VEGF作用于EPCs的表面受体VEGFR2,从而加速EPCs的动员和迁移入血。

Objective： To observe the influence of electroacupuncture pretreatment to endothelial progenitor cells （EPCs） and vessel endothelinm growth factor （VEGF） in marrow and peripheral blood, and to investigate the mechanism of electroacupuncture pretreatment in relieving brain ischemia-reperfusion injury. Method：The rats were divided into treatment group, model group, sham operation group and normal group by sortition randomization method. After cerebral ischemia-reperfusion models were made, flow cytometer was used to detect EPCs in marrow and peripheral blood, and VEGF in marrow and serum were detected by ELISA method, at the 12^th, 24^th and 48^th respectively. Result： Cerebral ischmeia-reperfusion rats＇ contents of EPCs and VEGF in bone marrow and peripheral blood were higher than that in normal rats; The contents of EPCs and VEGF in bone marrow and peripheral blood of treatment group were higher than that of unpretreatment group; and the longer after the electroacupuncture pretreatment, the more the contents of EPCs and VEGF increase. Conclusion： Electroacupuneture pretreatment could increase the contents of EPCs and VEGF in bone marrow and peripheral blood of cerebral ischemia-reperfusion injury rats. Electroacupuncture pretreatment could through VEGF increasing promote the motivation and migration of EPCs.