酶联免疫法检测HBsAg和荧光定量PCR检测HBV-DNA的对比研究

Comparison Research of ELISA and PCR in Examining HBsAg and HBV-DNA

目的对酶联免疫法检测HBsAg和荧光定量PCR检测HBV-DNA结果行对比研究,探讨其临床意义。方法对45例HBsAg阴性的慢性乙型肝炎HBV感染患者采用ELISA检测HBVM(HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc),同时采用PCR对其血清HBV-DNA进行定量分析;对照组为同期慢性乙型肝炎患者。结果45例HBsAg阴性慢性乙型肝炎患者中,HBV-DNA阳性率为24.44%。结论ELISA法检测HBV是不全面的,但PCR只能检出复制状态的病毒,难以表达非复制状态的感染,因而用PCR方法检测HBV-DNA也不能完全代替血清标志物的检测。建议必要时两者结合加强对患者的检测,以避免临床不必要的漏检。

Objective To compare the clinical value and reliability of ELISA （enzyme linked immunosorbent assay） and PCR（polymerase chain reaction）in examining HBsAg（hepatitis B surface antigen）and HBV-DNA（hepatitis B virus-DNA）. Methods Examining HBVM （HBsAg,Anti-HBs, HBeAg,Anti-HBe,Anti-HBc）by ELISA and HBV-DNA by PCR of 45 HBV patients. Results Of all the 45 HBV patients, the positive rate of HBV-DNA was 24.44%. Conclusion ELISA and PCR can not correctly examine the results and in clinical we should examine blood by both.