天晴甘平减轻急性肝衰竭小鼠肝细胞损伤的机制

Mechanism of diammonium glycyrrhizinate entericcoated capsules in alleviating hepatocytic injury in mice with acute liver failure

目的:探讨天晴甘平减轻急性肝衰竭肝细胞损伤的机制.方法:54只健康雌性昆明小白鼠随机分为实验组、对照组、正常对照组3组,每组18只.腹腔注射D-氨基半乳糖(D-galn)和脂多糖(LPS)制作急性肝衰竭模型.注射前3h,实验组给予天晴甘平灌胃,分别于注射后不同时间点用彗星实验检测肝细胞核DNA的损伤.结果:实验组和对照组的Olive尾矩值在0.5h即开始增大,且随时间延伸逐渐增加,与正常对照组比较,差异均具显著意义(0.5h:3.95±1.42,4.79±1.62vs0.95±0.56,均P<0.05);实验组与对照组比较,实验组Olive尾矩值明显变小,差异亦具显著意义(0.5-8h:10.81±2.85vs19.36±3.95,P<0.05).结论:急性肝衰竭小鼠肝细胞损伤的发生机制之一为肝细胞核DNA的损伤,天晴甘平具有减轻急性肝衰竭小鼠肝细胞核DNA损伤的作用.

AIM： To investigate the mechanism of Diammonium Glycyrrhizinate Enteric-coated Capsules in alleviating hepatocytic injury in mice with acute liver failure. METHODS： Fifty-four healthy female Kunming white mice were randomly divided into 3 groups： experimental group, control group, normal control group （n = 18 in each group）. The mice were injected intraperitoneally with D-Galactosamine （D-galn） and lipopolysaccharide （LPS） to create the mouse model of acute liver failure. Three hours prior to the injection, the experimental group were intragastrically administered Diammonium Glycyrrhizinate Enteric- coated Capsules. Comet assay was performed to detect DNA damages of hepatocytes of the mice at different time points after injection. RESULTS： Oliver tail moment values of both experimental group and control group began to increase over time 0.5 h after the injection and they were significantly greater than that of the normal control group （0.5 h： 3.95± 1.42, 4.79± 1.62 vs 0.95 ± 0.56, both P 〈 0.05）. The Oliver tail moment of the experimental group was significantly less than that of control group （0.5-8 h： 10.81 ± 2.85 vs 19.36 ± 3.95, P 〈 0.05）. CONCLUSION： One cause for the damages of hepatocytes of mice with acute liver failure may be the DNA damage of their nuclei, and Diammonium Glycyrrhizinate Enteric-coated Capsules can alleviate the damage to hepatocyte nuclear DNA in mice with acute liver failure.