高效液相色谱-蒸发光散射法测定南瓜中果糖、葡萄糖、及蔗糖含量

Determination of Fructoes, Glucose and Sucrose in Pumpkin by High Performance Liquid Chromatography-Evaporative Light Scattering Detection

目的:建立简便、准确、灵敏的测定南瓜中果糖、葡萄糖及蔗糖含量的方法。方法:样品经热水提取后,使用KromasilNH2(250mm×4.6mm,5μm)柱分离,以乙腈-水(80:20,V/V)为流动相,使用蒸发光散射检测器检测。结果:果糖、葡萄糖、蔗糖、麦芽糖及乳糖的线性范围分别为0.5～3、0.75～5、0.5～5、1～10、1～10g/L,检出限分别为1.90、2.40、1.80、2.50、2.60μg/L,样品的平均加标回收率分别为99.0%、97.1%、98.9%、99.5%、102.4%,相对标准偏差分别为1.02%、1.41%、1.27%、1.72%、1.66%。市售5种南瓜中糖果、葡萄糖、蔗糖的实际测定结果分别为1.05%～3.80%、0.823%～4.49%、0.090%～5.19%结论:该方法操作简便、快速、准确,用于实际样品的测定,结果令人满意。

Objective： To develop a simple, accurate and sensitive method for determination of fructose, glucose, sucrose, maltose and lactose in pumpkin. Methods： The sugars in pumpkin were extracted with hot water, separated on Kromasil NH2 column （250 mm × 4.6 mm, 5 μm） using acetonitrile and water （80：20, VN） as the mobile phase, and examined using an evaporative light scattering detector. Results： The linear ranges of fructose and glucose, sucrose, were maltose and lactose 0.5 to 3 g/L, 0.75 to 5 g/L, 0.5 to 5 g/L, 1 to 10 g/L and 1 to 10 g/L respectively. Their detection limits were 1.90, 2.40, 1.80, 2.50 and 2.60 μg/L, respectively. The average spike recoveries were 99.0%, 97.1%, 98.9%, 99.5% and 102.4%, respectively, and the relative standard deviations were 1.02%, 1.41%, 1.27%, respectively. The determination results of fructose, glucose and sucrose in five saled pumpkin samples were 1.05% to 3.80%, 0.823% to 4.49%, and 0.090% to 5.19%, respectively, while no maltose and lactose were found in these pumpkin samples. Conclusion： This method is simple, fast and accurate, and can be applied to the determination of fructose, glucose and sucrose in pumpkin with satisfactory results.