固相萃取高效液相色谱法测定猪肉中六种苯并咪唑类药物残留的研究

Determination of Six Benzimidazoles Residues in Pork by SPE-HPLC

建立了高效液相色谱法(HPLC)测定猪肉中硫氧苯唑、噻苯咪唑、康苯咪唑、甲苯咪唑、丙硫咪唑和硫苯咪唑残留量的检测方法。样品用乙酸乙酯提取,HLB固相萃取小柱净化,采用高效液相色谱分离,二极管阵列检测器(PDA)检测,外标法定量。结果表明:标准校准曲线在20～1000ng/ml的范围内呈良好线性关系,相关系数r>0.99。样品中硫氧苯唑、噻苯咪唑、康苯咪唑、甲苯咪唑、丙硫咪唑和硫苯咪唑的添加水平分别为0.05、0.1、0.15mg/L时,样品加标回收率为68.5%～83.7%,变异系数在1.6～7.3之间。本法简便、快速、定量准确、精密度高,适用于猪肉中苯并咪唑类药物残留量的检测。

Pork sample was extracted with ethyl acetate and purified by SPE- HLB cartridge. The analytes were further separated by HPLC and determined using PDA detector and quantified using external standard method. The results showed that there were good linear relationships between the peak areas and the concentrations of six benzimidazoles in the range of 20 to 1000 ng/ml （r 〉 0.99）. The average spike recoveries of six benzimidazoles were 68.5% to 83.7% at the spiked levels of 0.05, 0.1 and 0.15 mg/L for blank sample, and the precision RSDs were 1.6 to 7.3.