茶多酚的流动注射-共振光散射法测定

Determination of Tea Polyphones by Flow Injection Resonance Light Scattering Method

为了建立测定茶多酚(TP)的流动注射-共振光散射分析的新方法,在pH7的NaAc-NH4Ac缓冲溶液中,通过测定PbTP微粒的共振散射光强来定量测定TP的量。在此利用TP与Pb2+生成的沉淀反应,建立了流动注射-共振光散射联用技术测定TP的新方法。测定TP的线性范围为20～400μg/ml,方法的检出限为6.62μg/ml。建立的分析方法简便、试剂少、线性范围宽,可用于茶叶中TP含量的测定。

To establish a new flow injection resonance light scattering method for the determination of tea polyphones （TP）, PbTP was determined by resonance light scattering method in acetic acid buffer medium at pH 7. The amount of TP can be determined directly by mensuring the resonance light scattering intensity of PbTP that is precipitated by reaction of TP with Pb （II）, and thus a flow injection analysis-resonance light scattering method was proposed to determine TP. The linear range of determination was 20 to 400 μg/ml. The detection limit was 6.62 μg/ml. This method was simple, consumed less reagent and had wide linear range. It can be applied to the determination of TP in tea.