摘要
本实验采用戊二醛方法合成庆大霉素的免疫原,利用凝胶电泳对免疫原进行鉴定。使用该免疫原免疫兔子,得到抗血清测出效价高于512000,对抗血清进行纯化测出检测限达0.1ng/ml,建立庆大霉素的免疫检测方法。
An immunogen, gentamicin-bovine serum albumin （BSA）, was prepared by glutaraldehyde coupling method, and was identified by SDS-PAGE. New Zealand white rabbit was immunized with this immunogen three times. After immunization the antiserum with the titer of higher than 512000 was obtained. After purified by salting-out with ammonium sulfate, the obtained anti- gentamicin antibody was used to develop an indirect enzyme-linked immunosorbent assay （ELISA） for detection of gentamicin, with the detection limit of 0.1 ng/ml.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第10期242-244,共3页
Food Science
