趋化因子受体CXCR4在甲状腺乳头状癌的表达及临床意义

Expression and Clinical Significance of CXCR4 in Papillary Thyroid Cancer

目的:探讨趋化因子CXCR4在甲状腺乳头状癌中的表达及其与临床病理学参数的相关性。方法:利用半定量RT-PCR和免疫组织化学法分别检测72例新鲜甲状腺乳头状癌组织及52例甲状腺乳头状癌石蜡标本中CXCR4mRNA及蛋白的表达情况。结果:甲状腺乳头状癌组织中CXCR4mRNA及蛋白的阳性表达率分别为77.8%(56/72)、76.6%(95/124),而甲状腺良性病变组织及正常甲状腺组织均无表达,差异具有统计学意义(P<0.01);在甲状腺乳头状癌组织中,CXCR4mRNA表达与蛋白表达成正相关关系(r=0.714,P<0.01)。单因素分析显示CXCR4的蛋白表达与年龄、性别、肿瘤大小无关(P>0.05),但与肿瘤侵犯程度、淋巴结转移、远处转移、临床分期密切相关(P<0.05),且CXCR4蛋白阳性表达组的预后显著差于CXCR4蛋白阳性表达组(P<0.05)。结论:CXCR4阳性表达的甲状腺乳头状癌具有较高侵袭转移潜能,是预后不良的指标之一,CXCR4可作为抑制甲状腺乳头状癌侵袭转移的有效靶点。

Objective： To investigate the expression of CXCR4 in papillary thyroid cancer and its clinical significance. Methods： CXCR4 mRNA was detected in 72 fresh tumor tissues of papillary thyroid cancer using semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and CXCR4 protein was examined in 52 formalin-fixed tissues by immunohistochemistry. Results： The expression of CXCR4mRNA and protein in the papillary thyroid cancer was positively correlated and was significantly higher than that in the control group （P〈0.05）. Univariate analysis showed that the expression of CXCR4 was not significantly correlated with age, gender and the size of the tumor （P〉0.05）. The expression of CXCR4 was correlated with the degree of tumor invasion, lymph node metastasis, distant metastasis and clinical stage （P〈0.05）. The survival rate in the group with negative CXCR4 expression was significantly higher than that in the group with positive CXCR4 expression （P〈0.05）. Conclusion： The positive expression of CXCR4 indicates more potentiality of metastasis and invasion. CXCR can be an index of unfavorable prognosis and a new target to restrain the metastasis.