摘要
通过单因素实验和正交实验,对巴氏醋杆菌产乙醛脱氢酶的发酵培养基进行了优化,确定了培养基最适初始pH。得到优化培养基组分为:硫酸铵2.5g/L,磷酸氢二钾0.75g/L,硫酸镁0.2g/L,磷酸二氢钠0.5g/L,氯化钙0.1g/L,乳酸钠1.25%。发酵最适初始pH6.0。使用优化了的培养基可使生物量达到3.5g/L,粗酶活达到26U/mL。
The effects of different carbon and nitrogen sources on the biomass and acetaldehyde dehydrogenase activity of Acetobacter pasteurianus were studied by single-factor and orthogonal design experiments. The optimal medium contained 2.5 g/L (NH4)2SO4, 0.75 g/L K2HPO4, 0.2 g/L MgSO4, 0.5 g/L NaH2:PO4, 0.1 g/L CaCl2, and 1.25% sodium lactate. The best initial pH value is 6.0. After cultured in the optimized medium, the biomass of A. pasteurianus was up to 3.5 g/L, and the acetaldehyde dehydrogenase activity could reach 26 U/mL.
出处
《食品科技》
CAS
北大核心
2009年第5期6-9,共4页
Food Science and Technology
基金
湖北省国际科技合作重点项目(2006CA013)
湖北省科技攻关计划(2007AA201C27,2007AA301B24)
关键词
巴氏醋杆菌
乙醛脱氢酶
生物量
优化
Acetobacter pasteurianus
acetaldehyde dehydrogenase
biomass
optimization