巴氏醋杆菌产乙醛脱氢酶的培养基优化

Optimization of cultivation media of Acetobacter pasteurianus for acetaldehyde dehydrogenase

通过单因素实验和正交实验,对巴氏醋杆菌产乙醛脱氢酶的发酵培养基进行了优化,确定了培养基最适初始pH。得到优化培养基组分为:硫酸铵2.5g/L,磷酸氢二钾0.75g/L,硫酸镁0.2g/L,磷酸二氢钠0.5g/L,氯化钙0.1g/L,乳酸钠1.25%。发酵最适初始pH6.0。使用优化了的培养基可使生物量达到3.5g/L,粗酶活达到26U/mL。

The effects of different carbon and nitrogen sources on the biomass and acetaldehyde dehydrogenase activity of Acetobacter pasteurianus were studied by single-factor and orthogonal design experiments. The optimal medium contained 2.5 g/L （NH4）2SO4, 0.75 g/L K2HPO4, 0.2 g/L MgSO4, 0.5 g/L NaH2：PO4, 0.1 g/L CaCl2, and 1.25% sodium lactate. The best initial pH value is 6.0. After cultured in the optimized medium, the biomass of A. pasteurianus was up to 3.5 g/L, and the acetaldehyde dehydrogenase activity could reach 26 U/mL.