摘要
通过盐析、透析、阴离子交换层析等技术从马铃薯提取淀粉后的废液中分离纯化出了蛋白质酶抑制剂,并且通过对实验条件优化,准确地测定了蛋白酶抑制因子对胰蛋白酶的抑制能力。在实验条件下,所用胰蛋白酶的活力单位为110U/mg,加入2mg/mL的马铃薯蛋白质提取物以后,胰蛋白酶的活力单位降为26.67U/mg,蛋白酶抑制剂对胰蛋白酶的抑制作用较为显著,其抑制率可达75.76%。
Protease inhibitors were separated from potato waste through several treatments including salting out, dialysis, and anion exchange chromatography. By using the optimistic conditions, the activity of protease inhibitors was measured accurately. Under the given conditions, adding the solution of potato protein extractives with the concentration at 2 mg/mL to trypsin solution resulting in trypsin bioactivity fell down from 110 U/mg to 26.67 U/mg. It is demonstrated that the potato protein extractives displayed obvious inhibitory effect on trypsin, and the inhibitory rates Could reach to 75.76%.
出处
《食品科技》
CAS
北大核心
2009年第5期188-190,195,共4页
Food Science and Technology
基金
国家科技支撑计划项目(2007BAD49B00)
