鱿鱼眼透明质酸的提取工艺研究

Study on the extraction technology of hyaluronic acid from squid eyes

对鱿鱼眼提取透明质酸的方法进行了较系统的研究,实验采用水提醇沉、等电点法脱蛋白质与超滤相结合的加工工艺,运用正交实验法对提取工艺参数进行优化,并对产品的质量指标进行了检验。结果表明,最佳提取工艺参数为:浸提次数为3次,浸提时间为3h,料液比为1∶6,等电点pH4脱蛋白质。在此条件下,制备的透明质酸为白色粉末,得率为2.96%。所得成品含葡萄糖醛酸33.45%,氨基葡萄糖29.81%,蛋白质3.06%。光谱分析法结构鉴定结果表明它的主要成分为透明质酸。

This article introduced a method of preparation hyaluronic acid from the squid eyes, the experimental procedures were performed by aqueous extraction-alcohol precipitation, isoelectric precipitation and Ultrafiltration, the technological parameter was optimized by orthogonal experimental design, and its physical and chemical characteristics were also determined. The result showed that the optimal technological obtained are extraction 3 times, time 3h and solvent ratio 1：6; Isoelectric point pH=4. Under this echnological conditions, the white powder of hyaluronic acid is obtained at yield rate 2.96%. It contains 33.45% of glucuromic acid glucosam, 29.81% of amino-glucose and 3.06% of protein. Being identified by spectroscopic analysis, the main content of the product is hyaluronic acid.