斜带髭鲷后肠、头肾和脾脏中肥大细胞超微结构观察

Ultrastructural observations on the mast cells in hindgut,head kidney and spleen of Hapalogenys nitens

应用电镜技术研究了斜带髭鲷的后肠、头肾及脾脏中肥大细胞的超微结构。结果显示:在所研究的器官组织中均发现有肥大细胞,但以后肠和头肾分布较多;这些肥大细胞的表面具有胞质突起或表面折叠。根据其特征性胞浆颗粒所含特殊亚结构的不同,可初步认为斜带髭鲷肥大细胞可能存在Ⅰ型和Ⅱ型两种亚型。Ⅰ型肥大细胞主要分布于后肠粘膜固有层、粘膜下层和头肾的结缔组织中,其特征性胞浆颗粒具有微粒样特殊亚结构;Ⅱ型肥大细胞仅分布于头肾和脾脏的结缔组织中,其特征性胞浆颗粒则具有漩涡样、线团样、蜂巢样、细粒状、无定形和混合型等多种晶格状特殊亚结构。同时,这两种亚型肥大细胞均存在脱颗粒现象。由观察结果可知,斜带髭鲷后肠、头肾及脾脏中存在大量的肥大细胞,并且其特征性胞浆颗粒具有精细的特殊亚结构。

The mast ceils in hindgut, head kidney and spleen of Hapalogenys nitens were investigated by using transmission electron microscopy. The results showed that mast cells possessing cytoplasmic processes or surface folds were found in all the organs studied, but were especially abundant in hindgut and head kidney. The mast cells perhaps could be classified into two major subtypes by the special substructures of their characteristic cytoplasmic granules and referred to as type Ⅰ and type Ⅱ mast cells. The cytoplasmic granules with tiny particulate substructure were characteristic of the type Ⅰ mast cells, which distributed mainly in the lamina propria and submucosa layers of the hindgut and in the connective tissues of the head kidney. And the type Ⅱ mast cells locating only in the connective tissues of the head kidney and spleen were characterized by their cytoplasmic granules exhibiting a variety of crystalline substructural patterns, such as whorl, thread, honeycomb, particulate, amorphism, and mixture of these substructural patterns. The degranulation was also found in the two subtypes of mast cells. Our results demonstrate that numerous mast cells containing characteristic cytoplasmic granules with delicate special substructures are present in hindgut, head kidney and spleen of Hapalogenys nitens.