一个水稻分蘖角度突变体tac2的遗传分析和基因初步定位

Genetic Analysis and Gene Mapping of a Rice Tiller Angle Mutant tac2

水稻散生突变体tac2是以恢复系缙恢10号为材料经甲基磺酸乙酯(EMS)化学诱变所得。该突变体苗期表型正常,分蘖期株型松散,分蘖角度较野生型显著增大,株高明显降低。外源赤霉素处理可以使该突变体株高恢复,但分蘖角度不受影响。遗传分析表明该突变性状受1对隐性主效基因控制。利用分子标记将该基因初步定位于第9染色体上的RM3320与RM201之间,遗传距离分别为19.2和16.7 cM。

A tiller angle controlling 2（tac2） mutant was obtained by ethyl methanesulfonate（EMS） mutagenesis from a restorer line Jinhui 10. At the seedling stage, tac2 displayed normal phenotype and its tiller angle significantly increased at the tillering stage compared with wild type. A preliminary physiological research indicated that the mutant was sensitive to gibberellic acid. TAC2 and TAC1 maybe control the tiller angle in the same way. Genetic analysis showed that the mutant trait was controlled by a major recessive gene located at chromosome 9 using SSR markers. The genetic distances between TAC2 and its nearest markers RM3320 and RM201 were 19.2 and 16.7 cM, respectively.