香菇B.交配型位点的分子遗传学结构研究I．信息素受体和信息素前体编码基因的克隆和序列分析

Molecular genetic structure of the B mating-type locus of Lentinula edodes Ⅰ.Cloning and sequence analysis of pheromone receptor gene and pheromone precursor gene

本研究结合简并PCR和染色体步行两种方法研究了香菇135菌株的交配型B位点的分子遗传学结构。从135菌株的原生质体单核体1号菌株中获得了1个信息素受体编码基因LErcb1-B1和1个信息素前体编码基因LEphb1-B1。经序列比对分析,香菇的信息素受体LErcb1-B1序列与灰盖鬼伞和裂褶菌的信息素受体之间具有同源性,经SOSUI软件分析该序列具有7次跨膜结构特征。信息素前体LEphb1-B1具有CaaX基序特征。

The degenerate PCR and the chromosome walking technique were used to reveal the molecular genetics structure of B mating-type locus in strain No. 135 of Lentinula edodes. A pheromone receptor gene LErcbl-B1 and a pheromone precursor gene LEphbl-B1 were obtained from the protoplast monokaryon strain No. 1 of L. edodes strain No. 135. The pheromone receptor Lercbl-B1 of L. edodes had the homology with these pheromone receptors from Coprinus cinereus and Schizophyllum commune based on alignment, and was seven transmembrane protein following the analysis using the SOSUI software. The predicted pheromone precursor LEphb 1-B 1 contains the C-terminal CaaX motif.