裂蹄木层孔菌子实体水提物诱导HepG2细胞凋亡的初步研究

Water extract of Phellinus linteus fruiting body induces apoptosis of HepG2 cells

研究裂蹄木层孔菌子实体水提物(WEPL)对人类克隆肝癌细胞系HepG2生长的作用。用裂蹄木层孔菌子实体水提物处理HepG2细胞后,噻唑蓝法(MTT法)可见浓度和时间依赖性抑制细胞增殖;电镜下观察凋亡小体的出现,流式细胞仪技术显示Annexin-Ⅴ染色呈阳性,都证明了HepG2细胞发生了凋亡。RT-PCR和Western Blot分析证实WEPL刺激Bax表达量上调、Bcl-2表达量下调进而诱导了细胞凋亡。结果表明WEPL诱发的克隆人类肝癌细胞系HepG2的细胞凋亡可能是通过上调Bax、下调Bcl-2活性来实现的。

The effect of water extracts ofPhellinus linteus fruit bodies （WEPL） on the growth of HepG2 human colon cancer cells was investigated. Methylthiazolyldiphenyl-tetrazolium bromide （MTT） assay showed that the inhibition ratio of HepG2 cells treated with WEPL increased dose- and time- dependently. Microscopic observation proved the occurrence of apoptotic bodies. Flow cytometry detection showed the cells were positively stained by Annexin-V. These were the hallmark features of apoptosis. The mRNA expression level and protein level of Bax were adjusted up while that of Bcl-2 were adjusted down. It seems possible that WEPL induces HepG2 cell apoptosis through down-regulating the expression of Bcl-2 gene and up-regulating the expression of Bax gene.