舒巴坦与米诺环素对多药耐药鲍氏不动杆菌体外联合药敏研究

In-vitro Activity of Sulbactam in Combination with Minocycline Against Multidrug-resistant Acinetobacter baumannii

目的评价舒巴坦与米诺环素联合使用,对临床分离多药耐药鲍氏不动杆菌的体外抗菌效应。方法棋盘法设计,采用琼脂稀释法测定不同浓度组合的抗菌药物对110株临床分离的多药耐药鲍氏不动杆菌的最低抑菌浓度(MIC),并计算部分抑菌浓度(FIC)指数,以判定抗菌药物联合使用的效应。结果舒巴坦与米诺环素联合使用后,MIC50显著降低,联合抗菌效应为:协同效应占22%,累加效应占74%,无关效应占5%,无拮抗效应。结论舒巴坦与米诺环素联合使用对多药耐药的鲍氏不动杆菌表现为协同和累加作用,并以累加作用为主,无关作用较少,无拮抗作用。

OBJECTIVE To evaluate the antibacterial effect of sulbactam combined with minocycline against 110 multidrug-resistant Acinetobacter baurnannii strains. METHODS Checkerboard method was designed and agar dilution method was used for the MIC testing of sulbactam combined with minocycline against 110 multidrug- resistantA, baumannii strains, and the fractional inhibitory concentration （FIC） index was calculated according to MIC value. RESULTS The MIC50 of sulbactam was reduced significantly and the antimicrobial activities had been reinforced remarkably when combined with minocycline. The FIC results suggested that the main interaction be synergism （22%） and additivity （74%）, with only 5% non-effect and without antagonism. CONCLUSIONS Synergism and additivity are the main effects for subactam when combined with minocycline against multidrug-resistant A. baurnannii.