乙型肝炎患者血清HBV-LP、HBV-M、HBV-DNA之间的相关性分析

Relationship among Serum Hepatitis B Virus Large Envelope Protein,HBV-M and HBV-DNA in Patients with Hepatitis

目的检测患者血清乙型肝炎病毒外膜大蛋白(HBV-LP)、HBV-DNA、乙型肝炎病毒标志物(HBV-M),探讨HBV-LP对于反映体内乙型肝炎病毒复制的意义。方法采用荧光定量PCR方法对360份HBV感染血清的HBV-DNA进行检测,并采用酶联免疫吸附试验(ELISA)的方法对HBV-LP、HBV-M及HBV-DNA进行检测。结果大蛋白的检出结果与HBV-DNA的检出结果差异无统计学意义(P>0.05);不同HBV-M模式的HBV-DNA与HBV-LP的检出结果差异均无统计学意义(P>0.05);HBV-DNA拷贝数的对数值与HBV-LP表达具有相关关系(r=0.945,P<0.05)。结论HBV-LP能够反应HBV的复制情况,血清中HBV-LP的含量与HBV-DNA的拷贝数具有较好的相关性。

OBJECTIVE To explore the significance of hepatitis B virus large protein （HBV-LP） in the diagnosis of viral replication. METHODS Serum HBV DNA level was quantitatively detected using real-time polymerase chain reaction,and HBV-LP, HBV-M and HBV marker expressions were measured by enzyme linked immunosorbent assay （ELISA） in 360 infected serum samples. RESULTS There was no significant difference between the levels of HBV-DNA and HBV-LP （P〉0. 05）. The level of HBV-DNA and HBV-LP showed no significant difference among various patterns of HBV serological markers （P〉0. 05）, HBV-LP expression was markedly correlated with the logarithm of HBV-DNA level （r=0. 945, P〈 0. 05）. CONCLUSIONS There is a perfect correlation between the copies of HBV-DNA and the levels of HBV-LP. HBV-LP expression can reflect the replication of HBV.