荧光定量PCR对生殖道沙眼衣原体及解脲脲支原体检测结果分析

Detection of Chlamydia trachomatis and Ureaplasma urealyticum by Fluorescence Quantitative PCR

目的讨论非淋菌性生殖道炎症患者的沙眼衣原体(Ct)和解脲脲支原体(Uu)感染状况。方法采用荧光定量PCR法对1025例非淋菌性感染患者和30例健康体检者分别进行Ct和Uu检测。结果1025例非淋菌性感染患者中单一Ct阳性为156例,阳性率为15.22%,单一Uu阳性505例,阳性率为49.27%,Ct、Uu混合感染217例,阳性率为21.17%,总检出率为85.66%;Uu感染率女性明显多于男性,差异有统计学意义(2χ=104.56,P<0.01),而Ct感染率女性与男性差异无统计学意义(χ2=0.88,P>0.05),30例健康对照组中男性Ct和Uu结果全阴性;女性Uu结果5例阳性(16.67%),Ct结果全阴性。结论NGU患者中Uu感染率最高同时女性感染较男性要高,其次为Ct感染但感染率差异无统计学意义,在诊断非淋菌性感染时建议同时检测Ct和Uu以免漏诊。

OBJECTIVE To discuss the infection rate of Chlamydia trachomatis （Ct） and Ureaplasma urealyticum （Uu） in patients with non-gonoeoecal infection. METHODS Fluorescence quantitative PCR method was used on 1025 cases and 30 cases of NGU patients for Ct and Uu detection. RESULTS Of 1025 NGU patients, positive Ct alone accounted for 156 cases, the positive rate was 15.22%. 505 cases were separate Uu, the positive rate was 49.27%. Ct, Uu mixed in 217 cases, the positive rate was 21. 17%. The detection rate was 85. 66%. Uu infection rate in women was more than that in men （X^2 =104.56 P〈0.01）. There was no significant difference in Ct infection rate of women and men （X^2=0.88 P〉0. 05）. of control group, the Ct Uu Results negative. CONCLUSIONS In NGH patients, Uu is most common pathgen in man and woman. To diagnosis of NGU, Uu and Ct should be followed by Ct infection rate but no gender tested at the same time to avoid missed diagnosis.