摘要
目的:检测上皮型钙黏蛋白(E-cadherin)在结直肠癌、癌旁正常组织中的表达,并探讨其与临床病理特征之间的关系。方法:采用免疫组织化学En Vision两步法对120例结直肠癌组织及癌旁正常组织中E-cadherin蛋白进行检测。结果:癌组织和癌旁正常组织中(距肿瘤边缘5cm)E-cadherin的表达无明显差异(P=0.06)。中高分化癌组织中阳性上皮细胞百分比明显高于低分化癌组织。癌组织中上皮细胞膜表达丢失,而变为细胞浆表达或细胞膜细胞浆混合表达。这种细胞膜表达丢失与Dukes分期、肿瘤分化程度和转移发生相关,而与患者性别、肿瘤发生部位及肿瘤大小无关。结论:低分化癌组织中E-cadherin低表达可能与其侵袭特性有关;癌组织中上皮细胞膜表达丢失与性别、肿瘤大小和位置无关。
Objective：This study was designed to investigate the expression of E-cadherin,a calcium-dependent cell-cell adhesion molecule in colorectal carcinoma.The relation of their expression with the clinicopathological features of this disease was also to be established.Methods：Immunohistochemical analysis for E-cadherin was carried out in formalin-fixed,paraffin-embedded sections of neoplastic colorectal tissues and non-neoplastic ones adjacent to the lesion from 120 patients who underwent surgery.Expression of this antigen in normal and malignant epithelium was compared.Results：The expression of E-cadherin showed no difference between tumor and normal tissues（P=0.06）.The percentage of expression in epithelial cells of well or moderately differentiated tumors was significantly higher than low differentiated tumors.Loss of normal membranous expression and the presence of cytoplasmic and mixed staining were found frequently in tumor tissues.This loss of membranous expression related with Dukes＇ stage,tumor grade and metastasis.However,it did not correlate with sex,site or tumor size.Conclusion：This study suggests that the lower expression of E-cadherin in less differentiated tumors and tumors which metastasis occurred may explain their aggressive nature,although loss of membranous expression was not significantly correlated to sex,size and site of the tumor.
出处
《临床肿瘤学杂志》
CAS
2009年第4期354-357,共4页
Chinese Clinical Oncology
